Fan Jiangang, Tang Yuedi, Liang Chuanyu
Department of Otorhinolaryngology, People's Hospital of Sichuan Province, Chengdu, 610072, China.
Lin Chuang Er Bi Yan Hou Tou Jing Wai Ke Za Zhi. 2009 Nov;23(22):1038-43.
To explore the nature of voltage dependent ion channels and basic electrophysiological characteristics of cochlear spiral ganglion neurons of apical and basal turn by patch clamp techniques of whole cell configure on murine spiral ganglion neurons.
Different voltage dependent ionic currents were recorded with patch clamp techniques of whole cell configure on the condition of different internal electrode solution, blockers and stimulus protocol.
Inward sodium channel current (I(Na)), hyperpolarization-activated inward cationic current (Ih), outward delay rectification potassium current (I(K)) and outward transient potassium current (I(A)) were recorded ,significant difference of electrophysiological characteristics of I(A) and I(K) was found between apical and basal turns (P < 0.05).
Various ionic currents are recorded, which shows that spiral ganglion neurons have the base of ionic channels to complete formation,conduction and modulation of action potential for auditory information transduction, the difference of electrophysiological characteristics between apical and basal turns contributes to the course of hearing formation.
采用全细胞膜片钳技术，探讨小鼠螺旋神经节神经元顶回和底回电压依赖性离子通道的性质及基本电生理特性。
在不同的细胞内电极液、阻滞剂和刺激方案条件下，采用全细胞膜片钳技术记录不同的电压依赖性离子电流。
记录到内向钠通道电流（I(Na)）、超极化激活内向阳离子电流（Ih）、外向延迟整流钾电流（I(K)）和外向瞬时钾电流（I(A)），顶回和底回I(A)和I(K)的电生理特性存在显著差异（P < 0.05）。
记录到多种离子电流，表明螺旋神经节神经元具有离子通道基础，以完成听觉信息转导中动作电位的形成、传导和调制，顶回和底回电生理特性的差异有助于听觉形成过程。
