Zhu Jinge, Dhimitruka Ilirian, Pei Dehua
Department of Chemistry and Ohio State Biochemistry Program, The Ohio State University, 100 West 18th Avenue, Columbus, Ohio 43210, USA.
Org Lett. 2004 Oct 14;6(21):3809-12. doi: 10.1021/ol048404+.
[reaction: see text] 5-(2-Aminoethyl)dithio-2-nitrobenzoate (ADNB) reacts with free thiols with kinetics similar to those of Ellman's reagent but has dramatically improved stability under alkaline conditions, making it an excellent alternative to Ellman's reagent for the quantitation of thiol contents and enzymatic assays under basic pH conditions.
[反应：见正文] 5-（2-氨基乙基）二硫代-2-硝基苯甲酸酯（ADNB）与游离硫醇反应的动力学与埃尔曼试剂相似，但在碱性条件下稳定性显著提高，使其成为在碱性pH条件下定量硫醇含量和进行酶促测定时替代埃尔曼试剂的极佳选择。
