5-（2-氨基乙基）二硫代-2-硝基苯甲酸酯作为比埃尔曼试剂更耐碱的替代品。
5-(2-Aminoethyl)dithio-2-nitrobenzoate as a more base-stable alternative to Ellman's reagent.
作者信息
Zhu Jinge, Dhimitruka Ilirian, Pei Dehua
机构信息
Department of Chemistry and Ohio State Biochemistry Program, The Ohio State University, 100 West 18th Avenue, Columbus, Ohio 43210, USA.
出版信息
Org Lett. 2004 Oct 14;6(21):3809-12. doi: 10.1021/ol048404+.
PMID:15469355
Abstract
[reaction: see text] 5-(2-Aminoethyl)dithio-2-nitrobenzoate (ADNB) reacts with free thiols with kinetics similar to those of Ellman's reagent but has dramatically improved stability under alkaline conditions, making it an excellent alternative to Ellman's reagent for the quantitation of thiol contents and enzymatic assays under basic pH conditions.
摘要
[反应：见正文] 5-（2-氨基乙基）二硫代-2-硝基苯甲酸酯（ADNB）与游离硫醇反应的动力学与埃尔曼试剂相似，但在碱性条件下稳定性显著提高，使其成为在碱性pH条件下定量硫醇含量和进行酶促测定时替代埃尔曼试剂的极佳选择。
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