Mostefaoui Yakout, Bart Christian, Frenette Michel, Rouabhia Mahmoud
Groupe de recherche en écologie buccale, Faculté de médicine dentaire, Université Laval, Quebec City, Quebec, Canada G1K 7P4.
Cell Microbiol. 2004 Nov;6(11):1085-96. doi: 10.1111/j.1462-5822.2004.00420.x.
We investigated the involvement of oral epithelial cells via two cytokines (IL-6 and TNF-alpha) and one chemokine (IL-8) in local defences against live yeast (Candida albicans) and bacteria (Streptococcus salivarius) using an engineered human oral mucosa model. We report that the yeast changed from the blastospore to the hyphal form and induced significant tissue disorganization at later contact periods (24 and 48 h) compared to the bacteria. However, this effect did not reduce the viability or total number of epithelial cells. Gene activation analyses revealed that IL-6, IL-8 and TNF-alpha mRNA levels rose in tissues in contact with live C. albicans or S. salivarius. Gene activation was followed by an upregulation of protein secretion. IL-6 levels were higher after contact with C. albicans than with S. salivarius. IL-8 levels after contact with S. salivarius were higher than with C. albicans. Our study suggests that S. salivarius is more efficient at inducing proinflammatory mediator release than C. albicans. These results provide additional evidence for the contribution of oral epithelial cells to the inflammatory response against fungi and bacteria.
我们使用一种工程化的人类口腔黏膜模型，研究了口腔上皮细胞通过两种细胞因子（白细胞介素-6和肿瘤坏死因子-α）和一种趋化因子（白细胞介素-8）参与对活酵母（白色念珠菌）和细菌（唾液链球菌）的局部防御作用。我们报告称，与细菌相比，酵母在后期接触阶段（24小时和48小时）从芽生孢子转变为菌丝形态，并导致显著的组织紊乱。然而，这种效应并未降低上皮细胞的活力或总数。基因激活分析显示，与活的白色念珠菌或唾液链球菌接触的组织中，白细胞介素-6、白细胞介素-8和肿瘤坏死因子-α的mRNA水平升高。基因激活之后是蛋白质分泌的上调。与白色念珠菌接触后白细胞介素-6的水平高于与唾液链球菌接触后的水平。与唾液链球菌接触后白细胞介素-8的水平高于与白色念珠菌接触后的水平。我们的研究表明，唾液链球菌在诱导促炎介质释放方面比白色念珠菌更有效。这些结果为口腔上皮细胞对真菌和细菌炎症反应的贡献提供了额外的证据。
