Balbaa Mahmoud, El-Kersh Mohamed, Mansour Hamdy, Yacout Galila, Ismail Mohamed, Malky Ahmed, Bassiouny Khaled, Abdel-Monem Nihad, Kandeel Kamal
Department of Biochemistry, Faculty of Science, Alexandria University, Alexandria, Egypt.
J Biochem Mol Biol. 2004 Mar 31;37(2):223-8. doi: 10.5483/bmbrep.2004.37.2.223.
The levels of arylsulfatases A and B, alpha-amylase, aspartate transcarbamylase, and gamma-glutamyl transpeptidase were investigated during the infection of mice with schistosoma mansoni. This infection caused a significant (p < 0.001) increase in the activity of hepatic arylsulfatase B (ASB), aspartate transcarbamylases and gamma-glutamyl transpeptidase. A non-significant difference occurred for alpha-amylase (p < 0.3) and arylsulfatase A (p > 0.5) when compared to the control. The specific activity of hepatic ASB was progressively increased with the progression of the Schistosoma-infection. Moreover, the kinetic studies of hepatic ASB in Schistosoma-infection showed that a slight decrease in the value of K(m) and about a 40% increase in V(max) when compared to the control. In addition, the pH optimum of hepatic ASB was altered from 6 to 7 as a result of schistosomiasis. These observations suggest that there are schistosomiasis-associated changes of the catalytic and kinetic properties of hepatic ASB.
