大鼠小肠中磷酸二羟丙酮酰基转移酶的研究。部分氢化鱼油和氯贝丁酯的亚细胞定位及作用
Studies of dihydroxyacetone phosphate acyltransferase in rat small intestine. Subcellular localization and effect of partially hydrogenated fish oil and clofibrate.
作者信息
Ruyter B, Lund J S, Thomassen M S, Christiansen E N
机构信息
Institute for Nutrition Research, University of Oslo, Norway.
出版信息
Biochem J. 1992 Mar 1;282 ( Pt 2)(Pt 2):565-70. doi: 10.1042/bj2820565.
The subcellular localization of dihydroxyacetone phosphate acyltransferase (DHAPAT) activity in rat small intestine was investigated by Nycodenz-gradient centrifugation. We found that DHAPAT had a predominant peroxisomal distribution, with a separate enzyme activity located in the microsomal fraction, the same distribution as found in rat liver. The effect of feeding rats on a diet with 20% (w/w) partially hydrogenated fish oil (PHFO) or 0.3% clofibrate on the activity of DHAPAT in rat small intestine and liver was studied. Both 20% PHFO and 0.3% clofibrate gave a 1.8-fold stimulation of the specific activities of DHAPAT in peroxisomes of the small intestine, whereas in the liver 20% PHFO gave a 1.4-fold stimulation and 0.3% clofibrate a 1.6-fold stimulation of the total DHAPAT activities in the postnuclear supernatant. The specific activities of DHAPAT in liver were not affected.
通过 Nycodenz 密度梯度离心法研究了大鼠小肠中二羟基丙酮磷酸酰基转移酶（DHAPAT）活性的亚细胞定位。我们发现 DHAPAT 主要分布在过氧化物酶体中，在微粒体部分也有单独的酶活性，这与在大鼠肝脏中发现的分布相同。研究了用含 20%（w/w）部分氢化鱼油（PHFO）或 0.3%氯贝丁酯的饮食喂养大鼠对大鼠小肠和肝脏中 DHAPAT 活性的影响。20% PHFO 和 0.3%氯贝丁酯均使小肠过氧化物酶体中 DHAPAT 的比活性提高了 1.8 倍，而在肝脏中，20% PHFO 使核后上清液中总 DHAPAT 活性提高了 1.4 倍，0.3%氯贝丁酯使其提高了 1.6 倍。肝脏中 DHAPAT 的比活性不受影响。
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