Schultsz C, Qadri F, Hossain S A, Ahmed F, Ciznar I
Department of Medical Microbiology, Academical Medical Centre, Amsterdam, The Netherlands.
FEMS Microbiol Immunol. 1992 Jan;4(2):65-72. doi: 10.1111/j.1574-6968.1992.tb04972.x.
To study the secretory immune response after Shigella infection, the anti-lipopolysaccharide and anti-Shiga-toxin response in saliva, obtained from children with confirmed shigellosis and healthy children, were determined by enzyme-linked immunosorbent assay and by Western blot. Children with infection showed high titers compared to healthy controls. After Shigella dysenteriae type 1 infection a significant change in titer could be observed in a large number of cases, in contrast to Shigella flexneri infection. It appeared that, in children living in endemic areas, infection with one serotype can give a rise in antibody titer to another serotype. This could be ascribed to polyclonal B cell activation since children in endemic areas routinely show relatively high titers to Shigella antigens. We conclude that the dynamics of salivary anti-Shigella LPS and anti-Shiga-toxin in children with dysentery indicate that it can be applied to studies of immune response in shigellosis for epidemiological and vaccination purposes.
为研究志贺菌感染后的分泌性免疫反应，采用酶联免疫吸附测定法和蛋白质印迹法，对确诊为志贺菌病的儿童及健康儿童唾液中的抗脂多糖和抗志贺毒素反应进行了检测。与健康对照相比，感染儿童的滴度较高。与福氏志贺菌感染相比，在大量病例中可观察到1型痢疾志贺菌感染后滴度有显著变化。似乎在流行地区的儿童中，感染一种血清型可导致针对另一种血清型的抗体滴度升高。这可能归因于多克隆B细胞激活，因为流行地区的儿童通常对志贺菌抗原显示出相对较高的滴度。我们得出结论，痢疾患儿唾液中抗志贺菌脂多糖和抗志贺毒素的动态变化表明，它可用于志贺菌病免疫反应的研究，以用于流行病学和疫苗接种目的。
