Marco F, Jurado A, Jiménez de Anta M T
Servicio de Microbiología, IDIBAPS, Hospital Clínic, Barcelona.
Rev Esp Quimioter. 2004 Jun;17(2):169-76.
The Phoenix system (BD Diagnostic Systems), a rapid ID/AST system, was compared with the MicroScan WalkAway-40 system for accuracy of identification and antimicrobial susceptibility test results. The 327 bacterial isolates, were comprised of 191 Gram-negative bacilli (187 Enterobacteriaceae and 4 Aeromonas spp.) and 136 Gram-positive cocci (27 Staphylococcus aureus, 53 coagulase-negative staphylococci, 45 enterococci and 11 beta haemolytic streptococci). The overall rate of agreement between the two systems for species level identification was 95.8% and 96.3% for Gram-negative bacilli and Gram-positive cocci, respectively. Enterococcus and Streptococcus species both achieved a 100% rate of species level agreement. The genus level agreement was >99% overall. Arbitration of the 8 Gram-negative bacilli disagreements resolved with 7 in agreement with the Phoenix identification. For the 5 Gram-positive cocci disagreements, 3 resolved in agreement with Phoenix. Overall, 3688 antimicrobial/organism combinations were evaluated in both systems. For Gram-negative isolates, the rate of essential agreement for the MICs was 98.5%, while the categorical agreement rate was 95.9%. Arbitration of 13 Gram-negative disagreements resolved with 11 in agreement with the Phoenix system. For Staphylococcus spp. and Enterococcus spp. isolates, the essential agreement rates were 96.4% and 99% respectively. Categorical agreement rates for both genera were 94.7% and 96.1%, respectively. Arbitration of 5 staphylococci disagreements resolved with 2 in agreement with Phoenix system. Our results show that the Phoenix system is a rapid and reliable system for both identification and antimicrobial susceptibility testing of common clinical isolates.
将凤凰系统（BD诊断系统）这一快速鉴定/药敏试验系统与MicroScan WalkAway - 40系统在细菌鉴定准确性和抗菌药敏试验结果方面进行了比较。327株细菌分离株包括191株革兰氏阴性杆菌（187株肠杆菌科细菌和4株气单胞菌属细菌）以及136株革兰氏阳性球菌（27株金黄色葡萄球菌、53株凝固酶阴性葡萄球菌、45株肠球菌和11株β溶血性链球菌）。两种系统在菌种水平鉴定上的总体符合率，革兰氏阴性杆菌为95.8%，革兰氏阳性球菌为96.3%。肠球菌属和链球菌属菌种在菌种水平的符合率均达到100%。总体属水平符合率>99%。对8株革兰氏阴性杆菌的鉴定分歧进行仲裁，结果7株与凤凰系统的鉴定结果一致。对于5株革兰氏阳性球菌的鉴定分歧，3株与凤凰系统的鉴定结果一致。总体而言，两个系统共评估了3688种抗菌药物/细菌组合。对于革兰氏阴性分离株，最低抑菌浓度（MIC）的基本符合率为98.5%，类别符合率为95.9%。对13例革兰氏阴性鉴定分歧进行仲裁，11例与凤凰系统的结果一致。对于葡萄球菌属和肠球菌属分离株，基本符合率分别为96.4%和99%。两个菌属的类别符合率分别为94.7%和96.1%。对5例葡萄球菌鉴定分歧进行仲裁，2例与凤凰系统的鉴定结果一致。我们的结果表明，凤凰系统对于常见临床分离株的鉴定和抗菌药敏试验而言，是一个快速且可靠的系统。
