Department of Cell Biology and Biophysics, Faculty of Biology, University of Athens, Athens, Greece.
J Clin Microbiol. 2011 Sep;49(9):3284-91. doi: 10.1128/JCM.00182-11. Epub 2011 Jul 13.
Phoenix 100 and Vitek 2 (operating with the current colorimetric cards) are commonly used in hospital laboratories for rapid identification of microorganisms. The present meta-analysis aims to evaluate and compare their performance on Gram-positive and Gram-negative bacteria. The MEDLINE database was searched up to October 2010 for the retrieval of relevant articles. Pooled correct identification rates were derived from random-effects models, using the arcsine transformation. Separate analyses were conducted at the genus and species levels; subanalyses and meta-regression were undertaken to reveal meaningful system- and study-related modifiers. A total of 29 (6,635 isolates) and 19 (4,363 isolates) articles were eligible for Phoenix and colorimetric Vitek 2, respectively. No significant differences were observed between Phoenix and Vitek 2 either at the genus (97.70% versus 97.59%, P = 0.919) or the species (92.51% versus 88.77%, P = 0.149) level. Studies conducted with conventional comparator methods tended to report significantly better results compared to those using molecular reference techniques. Speciation of Staphylococcus aureus was significantly more accurate in comparison to coagulase-negative staphylococci by both Phoenix (99.78% versus 88.42%, P < 0.00001) and Vitek 2 (98.22% versus 91.89%, P = 0.043). Vitek 2 also reached higher correct identification rates for Gram-negative fermenters versus nonfermenters at the genus (99.60% versus 95.90%, P = 0.004) and the species (97.42% versus 84.85%, P = 0.003) level. In conclusion, the accuracy of both systems seems modified by underlying sample- and comparator method-related parameters. Future simultaneous assessment of the instruments against molecular comparator procedures may facilitate interpretation of the current observations.
菲尼克斯 100 和 Vitek 2（使用当前比色卡）常用于医院实验室快速鉴定微生物。本荟萃分析旨在评估和比较它们对革兰阳性和革兰阴性细菌的性能。检索了截至 2010 年 10 月的 MEDLINE 数据库以获取相关文章。使用反正弦变换，从随机效应模型得出了正确的鉴定率。在属和种水平上进行了单独分析；进行了亚分析和荟萃回归以揭示有意义的系统和研究相关的修饰剂。分别有 29 篇（6635 株）和 19 篇（4363 株）文章分别符合 Phoenix 和比色 Vitek 2 的要求。在属（97.70%对 97.59%，P=0.919）或种（92.51%对 88.77%，P=0.149）水平上，Phoenix 和 Vitek 2 之间没有显著差异。与使用分子参考技术的研究相比，使用常规比较方法的研究往往报告出显著更好的结果。与凝固酶阴性葡萄球菌相比，金黄色葡萄球菌的分类通过 Phoenix（99.78%对 88.42%，P<0.00001）和 Vitek 2（98.22%对 88.42%，P=0.043）都更为准确。Vitek 2 对革兰氏阴性发酵菌与非发酵菌的属（99.60%对 95.90%，P=0.004）和种（97.42%对 84.85%，P=0.003）水平的正确鉴定率也更高。总之，两个系统的准确性似乎都受到样本和比较方法相关参数的影响。未来对仪器进行同时评估与分子比较程序可能有助于解释当前的观察结果。
