Naas Thierry, Aubert Daniel, Vimont Sophie, Nordmann Patrice
Service de Bactériologie-Virologie, Hôpital de Bicêtre, Assistance Publique/Hôpitaux de Paris, Faculté de Médecine Paris-Sud, 78 rue du Général Leclerc, 94275 Le Kremlin-Bicêtre Cedex, France.
J Antimicrob Chemother. 2004 Nov;54(5):932-5. doi: 10.1093/jac/dkh446. Epub 2004 Oct 7.
To characterize the beta-lactamase gene content of Erwinia rhapontici.
The beta-lactamase gene was cloned, sequenced and expressed in Escherichia coli.
The cloned gene conferred a resistance pattern of an Ambler class C beta-lactamase in E. coli. The AmpC-type enzyme had a pI value of 8.6 and shared 62% amino acid sequence identity with that of Escherichia fergusonii. The ampC gene was associated with a regulatory ampR gene and beta-lactamase production was inducible.
This work provides further evidence of the molecular heterogeneity of beta-lactamases in Erwinia spp. and that plant-pathogenic enterobacterial species may constitute a reservoir of antibiotic resistance genes.
鉴定莱茵衣藻欧文氏菌的β-内酰胺酶基因含量。
克隆β-内酰胺酶基因，进行测序并在大肠杆菌中表达。
克隆的基因在大肠杆菌中赋予了安布勒C类β-内酰胺酶的耐药模式。AmpC型酶的pI值为8.6，与弗格森埃希氏菌的氨基酸序列同一性为62%。ampC基因与调控ampR基因相关，β-内酰胺酶的产生是可诱导的。
这项工作进一步证明了欧文氏菌属中β-内酰胺酶的分子异质性，以及植物致病性肠杆菌物种可能构成抗生素耐药基因的储存库。
