吲哚 - 3 - 甲醇激活细胞周期蛋白依赖性激酶抑制剂p15（INK4b）基因。

Indole-3-carbinol activates the cyclin-dependent kinase inhibitor p15(INK4b) gene.

作者信息

Matsuzaki Youichirou, Koyama Makoto, Hitomi Toshiaki, Kawanaka Mayumi, Sakai Toshiyuki

机构信息

Department of Molecular-Targeting Cancer Prevention, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kawaramachi-Hirokoji, Kamigyo-ku, Kyoto 602-8566, Japan.

出版信息

FEBS Lett. 2004 Oct 8;576(1-2):137-40. doi: 10.1016/j.febslet.2004.09.002.

DOI:10.1016/j.febslet.2004.09.002

PMID:15474025

Abstract

Indole-3-carbinol (I3C) is a naturally occurring compound found in vegetables such as broccoli and cauliflower, and has been shown to arrest human tumor cells in the G1 phase of the cell cycle. However, the molecular mechanism responsible for this effect has not been sufficiently elucidated. We report here that I3C activates the cyclin-dependent kinase (CDK) inhibitor p15INK4b gene through its promoter, accompanied by cell growth inhibition in HaCaT cells. Treatment with I3C almost did not affect the expressions of the other CDK inhibitors such as p19INK4d, p21WAF1 and p27Kip1. These results suggest that p15INK4b is an important molecular target of I3C among CDK inhibitors.

摘要

吲哚 - 3 - 甲醇（I3C）是一种天然存在的化合物，存在于西兰花和菜花等蔬菜中，已被证明可使人类肿瘤细胞停滞在细胞周期的G1期。然而，导致这种效应的分子机制尚未得到充分阐明。我们在此报告，I3C通过其启动子激活细胞周期蛋白依赖性激酶（CDK）抑制剂p15INK4b基因，同时在HaCaT细胞中抑制细胞生长。用I3C处理几乎不影响其他CDK抑制剂如p19INK4d、p21WAF1和p27Kip1的表达。这些结果表明，在CDK抑制剂中，p15INK4b是I3C的重要分子靶点。