呋喃妥因与霍乱弧菌DNA的体外相互作用。

In-vitro interaction between nitrofurantoin and Vibrio cholerae DNA.

作者信息

Mukherjee U, Chatterjee S N

机构信息

Biophysics Division, Saha Institute of Nuclear Physics, Calcutta, India.

出版信息

Chem Biol Interact. 1992 Mar;82(1):111-21. doi: 10.1016/0009-2797(92)90018-g.

DOI:10.1016/0009-2797(92)90018-g

PMID:1547512

Abstract

In-vitro interaction of nitrofurantoin with V. cholerae DNA resulted in a quenching and red spectral shift of the drug absorption pattern. Scatchard analysis revealed that the drug binding involved more than one processes and that the strongest mode of binding was characterised by an association constant (k) of 5.04 x 10(6) M-1 and the number of binding sites per nucleotide (n) of 0.015. Based on viscosity measurements, the mode of drug binding to DNA appeared to be through intercalation, the helix unwinding angle of supercoiled plasmid pBR322 DNA being 10 degrees. Nitrofurantoin binding to DNA resulted in an elevation of the thermal melting temperature (Tm) of DNA by 6 degrees C and inhibition of the action of DNase on DNA.

摘要

呋喃妥因与霍乱弧菌DNA的体外相互作用导致药物吸收模式的猝灭和红移光谱。斯卡查德分析表明，药物结合涉及多个过程，最强结合模式的特征是缔合常数（k）为5.04×10⁶ M⁻¹，每个核苷酸的结合位点数（n）为0.015。基于粘度测量，药物与DNA的结合模式似乎是通过嵌入，超螺旋质粒pBR322 DNA的螺旋解旋角为10度。呋喃妥因与DNA的结合导致DNA的热解链温度（Tm）升高6℃，并抑制DNase对DNA的作用。