van Hagen M A E, van der Kolk J, Barendse M A M, Imholz S, Leegwater P A J, Knol B W, van Oost B A
Department of Clinical Sciences of Companion Animals, Faculty of Veterinary Medicine, Utrecht University, P.O. Box 80.154, NL-3508 TD Utrecht, The Netherlands.
J Hered. 2004 Nov-Dec;95(6):526-31. doi: 10.1093/jhered/esh083.
The genetic basis of the white spotting pattern in Dutch boxer dogs is not known. We studied whether the segregation of white spotting in boxers follows a Mendelian inheritance pattern. Blood samples were collected, along with digital photographs in standard directions of (grand)parents (n=16) and offspring (n=52) from eight litters of Dutch boxers. In order to select heterozygous parents, we selected nonuniform litters, in which at least one puppy was extreme white. On the basis of criteria for the location, the extent of white spotting, and the mean percentage of pigmented area of the foot soles, we classified 10 dogs as solid colored, 27 as flashy, and 15 as extreme white. This was not a significant deviation from the expected 1:2:1 ratio. Because the flashy phenotype seems to be an intermediate between the two homozygotes, white spotting in the Dutch boxer can be considered to be due to a single gene effect, with incomplete dominance. We have evaluated candidate genes c-KIT (KIT) and EDNRB for segregation with white spotting phenotype in these litters. Using polymorphic markers, very near the KIT and EDNRB genes, we found that segregation of the white spotting pattern did not coincide with segregation of these polymorphic markers. Thus neither KIT nor EDNRB are likely to be responsible for white spotting in the Dutch population of boxers.
荷兰拳师犬白色斑点图案的遗传基础尚不清楚。我们研究了拳师犬白色斑点的分离是否遵循孟德尔遗传模式。采集了来自八窝荷兰拳师犬的（外）祖父母（n = 16）和后代（n = 52）的血样，并按照标准方向拍摄了数码照片。为了选择杂合子亲本，我们选择了非均匀毛色的一窝犬，其中至少有一只幼犬是极端白色的。根据白色斑点的位置、范围以及足底色素沉着区域的平均百分比标准，我们将10只犬分类为纯色，27只为花斑色，15只为极端白色。这与预期的1:2:1比例没有显著偏差。由于花斑色表型似乎是两种纯合子之间的中间类型，荷兰拳师犬的白色斑点可被认为是由单个基因效应导致的，具有不完全显性。我们评估了候选基因c-KIT（KIT）和EDNRB在这些窝犬中与白色斑点表型的分离情况。使用非常靠近KIT和EDNRB基因的多态性标记，我们发现白色斑点图案的分离与这些多态性标记的分离不一致。因此，KIT和EDNRB都不太可能是荷兰拳师犬种群中白色斑点的原因。
