Shirai Yoshinori, Ito Masao
Laboratory for Memory and Learning, Brain Science Institute, RIKEN, Wako, Saitama 351-0198, Japan.
J Neurocytol. 2004 May;33(3):297-307. doi: 10.1023/B:NEUR.0000044191.83858.f7.
Phospholipase A2 (PLA2) is a family of enzymes playing diverse roles in lipid signaling in neurons and glia cells. In this study, we examined the expression of subtypes of PLA2 in the cerebellum using immunolabeling and in situ hybridization methods. Two Ca2+-dependent cytosolic subtypes (cPLA2alpha and cPLA2beta), one Ca2+-independent cytosolic subtype (iPLA2), and two secretory subtypes (sPLA2IIA and sPLA2V) were detected in the cerebellum. cPLA2alpha is present in somata and dendrites of Purkinje cells, while sPLA2IIA is associated with the endoplasmic reticulum in perinuclear regions of Purkinje cell somata. iPLA2 is present in granule cells, stellate cells and also in the nucleus of Purkinje cells. In addition, cPLA2beta is localized in granule cells, and sPLA2V in Bergmann glia cells. These results provide an important basis for identifying functional roles of PLA2s in the cerebellum.
磷脂酶A2（PLA2）是一类在神经元和神经胶质细胞的脂质信号传导中发挥多种作用的酶。在本研究中，我们使用免疫标记和原位杂交方法检测了小脑内PLA2各亚型的表达情况。在小脑中检测到了两种钙离子依赖的胞质亚型（cPLA2α和cPLA2β）、一种钙离子非依赖的胞质亚型（iPLA2）以及两种分泌型亚型（sPLA2IIA和sPLA2V）。cPLA2α存在于浦肯野细胞的胞体和树突中，而sPLA2IIA与浦肯野细胞胞体核周区域的内质网相关。iPLA2存在于颗粒细胞、星状细胞以及浦肯野细胞的细胞核中。此外，cPLA2β定位于颗粒细胞中，而sPLA2V定位于伯格曼胶质细胞中。这些结果为确定PLA2在小脑中的功能作用提供了重要依据。
