Huyse Tine, Malmberg Göran, Volckaert Filip A M
Katholieke Universiteit Leuven, Laboratory of Aquatic Ecology, Ch. de Bériotstraat 32, B-3000 Leuven, Belgium.
Syst Parasitol. 2004 Oct;59(2):103-20. doi: 10.1023/B:SYPA.0000044427.81580.33.
Four Gyrodactylus species parasitising four closely related gobiid species in European coastal waters were studied and compared with G. arcuatus Bychowsky sensu Bychowsky & Poljansky (1953) from Gasterosteus aculatus . These were G. gondae n. sp. from Pomatoschistus minutus and P. lozanoi , G. flavescensis n. sp. from Gobiusculus flavescens , G. arcuatoides n. sp. from P. minutus and G. branchialis n. sp. from P. microps. Combined molecular and morphological analyses, as well as morphometric and statistical methods, were used. The ssrRNA V4 region and the complete ITS rDNA region were sequenced. Genetically the four new species are clearly distinct from G. arcuatus . From a morphological point of view, the haptoral hard parts of G. gondae n. sp., G. flavescensis n. sp. and G. arcuatoides n. sp. are related to those of G. arcuatus, while these parts of G. branchialis n. sp. are different, but related to those of G. quadratidigitus Longshaw, Pursglove & Shinn, 2003. For the latter two species, a new species group is formed. The V4 and ITS sequence analyses, however, indicate a close relationship between G. branchialis and the three G. arcuatus-like species.
对寄生于欧洲沿海水域四种近缘虾虎鱼的四种三代虫进行了研究，并与来自三刺鱼的拜霍夫斯基和波利亚尼斯基（1953年）所定义的弓形三代虫进行了比较。这些三代虫分别是：来自细纹斑尾虾虎鱼和洛氏斑尾虾虎鱼的贡氏三代虫新种；来自黄褐小虾虎鱼的黄褐三代虫新种；来自细纹斑尾虾虎鱼的类弓形三代虫新种；以及来自小鳍斑尾虾虎鱼的鳃三代虫新种。采用了分子与形态学相结合的分析方法以及形态测量和统计方法。对小亚基核糖体RNA（ssrRNA）的V4区域和完整的内转录间隔区核糖体DNA（ITS rDNA）区域进行了测序。从基因角度来看，这四个新种与弓形三代虫明显不同。从形态学角度来看，贡氏三代虫新种、黄褐三代虫新种和类弓形三代虫新种的后吸器硬体部分与弓形三代虫的相关，而鳃三代虫新种的这些部分则不同，但与朗肖、珀斯格洛夫和欣（2003年）描述的四方指三代虫的相关。对于后两个物种，形成了一个新的物种组。然而，V4和ITS序列分析表明鳃三代虫与三种类似弓形三代虫的物种关系密切。
