Pons Laurent, Ponnappan Usha, Hall Renée A, Simpson Pippa, Cockrell Gael, West C Michael, Sampson Hugh A, Helm Ricki M, Burks A Wesley
Department of Pediatrics, Division of Pediatric Allergy and Immunology, Duke University Medical Center, Durham, NC 27710, USA.
J Allergy Clin Immunol. 2004 Oct;114(4):915-21. doi: 10.1016/j.jaci.2004.06.049.
Allergen-specific immunotherapy (IT) is an effective therapeutic modality to prevent further anaphylactic episodes in patients with insect sting hypersensitivity and is being investigated for peanut allergy. So far, peanut-specific IT has been unsuccessful because of the side effects of therapy. Soybean seed storage proteins share significant homology with the respective peanut allergens.
This study was undertaken in mice to investigate whether specific doses of soybean would desensitize peanut-allergic mice.
C3H/HeJ mice were sensitized to peanut with 3 intraperitoneal (IP) injections of crude peanut extract. The mice were desensitized by IP injections with either crude peanut or soybean extract for 4 weeks, 3 times a week. Controls included placebo desensitization with PBS and naive mice. After 2 weeks of rest, mice were challenged IP with crude peanut extract. Thirty minutes later, symptom scores and body temperatures were recorded. Serum immunoglobulins, peanut-induced splenocyte proliferation, and secreted cytokines were measured before and after desensitization.
The clinical symptoms in the soybean- and peanut-desensitized animals were markedly reduced compared with the placebo-treated mice. Specific IgG1 levels to crude peanut were significantly lower in the soy IT group than in the peanut IT group. The cellular response to crude peanut was also downregulated in the soy IT group, as shown by decreased peanut-specific stimulation indices and a cytokine profile skewed toward a T H 1 response.
Soy IT can be used to desensitize/downregulate peanut-specific response in peanut-allergic mice and could provide a new therapeutic intervention for peanut allergy.
变应原特异性免疫疗法（IT）是预防昆虫叮咬过敏患者进一步发生过敏反应的一种有效治疗方式，目前正用于花生过敏的研究。到目前为止，由于治疗的副作用，花生特异性IT尚未成功。大豆种子储存蛋白与相应的花生变应原具有显著的同源性。
本研究在小鼠中进行，以调查特定剂量的大豆是否能使花生过敏小鼠脱敏。
用3次腹腔内（IP）注射粗花生提取物使C3H/HeJ小鼠对花生致敏。通过每周3次、共4周的IP注射粗花生提取物或大豆提取物使小鼠脱敏。对照组包括用PBS进行安慰剂脱敏的小鼠和未致敏小鼠。休息2周后，用粗花生提取物对小鼠进行IP激发。30分钟后，记录症状评分和体温。在脱敏前后测量血清免疫球蛋白、花生诱导的脾细胞增殖和分泌的细胞因子。
与安慰剂治疗的小鼠相比，大豆脱敏组和花生脱敏组动物的临床症状明显减轻。大豆IT组中针对粗花生的特异性IgG1水平显著低于花生IT组。大豆IT组中对粗花生的细胞反应也下调，表现为花生特异性刺激指数降低和细胞因子谱偏向于TH1反应。
大豆IT可用于使花生过敏小鼠的花生特异性反应脱敏/下调，并可为花生过敏提供一种新的治疗干预措施。
