Caserta Mary T, McDermott Michael P, Dewhurst Stephen, Schnabel Kenneth, Carnahan Jennifer A, Gilbert Lynne, Lathan Gladys, Lofthus Geraldine K, Hall Caroline B
Department of Pediatrics, University of Rochester School of Medicine, Rochester, New York, USA.
J Pediatr. 2004 Oct;145(4):478-84. doi: 10.1016/j.jpeds.2004.06.016.
To determine in healthy children after primary infection the persistence of human herpesvirus 6 (HHV6) DNA, the presence and frequency of HHV6 re-activation or re-infection, and the relationship of both to illness and the presence of human herpesvirus 7 (HHV7) infection.
Children 1 to 12 years of age with previous HHV6 infection were prospectively evaluated by HHV6 and HHV7 DNA polymerase chain reaction (PCR) and reverse transcription (RT)-PCR for HHV6. HHV6 plasma antibody titers were measured. Illnesses were recorded by diary, and physician records were reviewed.
HHV6 DNA was detected in >or=1 peripheral blood mononuclear cell (PBMC) samples in 89% of children. HHV6 reactivation and re-infection were detected by RT-PCR in 1.1% of samples. Detection of HHV6 DNA was intermittent in 76% of children and was not associated with cumulative rates of illness. Illness at a study visit was significantly associated with the absence of HHV6 and HHV7 DNA in PBMC samples and was not associated with HHV6 antibody titer or the presence of HHV6 DNA in saliva.
HHV6 DNA persists in most children intermittently following primary infection and is unrelated to illness. Reactivation of HHV6 occurs in healthy children without apparent illness.
确定原发性感染后健康儿童中人疱疹病毒6型（HHV6）DNA的持续存在情况、HHV6再激活或再感染的存在及频率，以及两者与疾病和人疱疹病毒7型（HHV7）感染存在情况的关系。
对1至12岁曾感染HHV6的儿童，采用HHV6和HHV7 DNA聚合酶链反应（PCR）及HHV6逆转录（RT）-PCR进行前瞻性评估。检测HHV6血浆抗体滴度。通过日记记录疾病情况，并查阅医生记录。
89%的儿童在≥1份外周血单个核细胞（PBMC）样本中检测到HHV6 DNA。通过RT-PCR在1.1%的样本中检测到HHV6再激活和再感染。76%的儿童中HHV6 DNA的检测呈间歇性，且与累积疾病发生率无关。研究访视时的疾病与PBMC样本中HHV6和HHV7 DNA的缺失显著相关，与HHV6抗体滴度或唾液中HHV6 DNA的存在无关。
原发性感染后，大多数儿童的HHV6 DNA间歇性持续存在，且与疾病无关。HHV6在无明显疾病的健康儿童中发生再激活。
