一项用于检测幼儿抗HIV-1 IgA的筛查试验。

A screening test for the detection of anti-HIV-1 IgA in young infants.

作者信息

Martin N L, Rautonen J, Crombleholme W, Rautonen N, Wara D W

机构信息

Department of Pediatrics, University of California, San Francisco.

出版信息

Immunol Invest. 1992 Feb;21(1):65-70. doi: 10.3109/08820139209069363.

DOI:10.3109/08820139209069363

PMID:1548047

Abstract

A simple dot blot screening test for anti-HIV-1 IgA in infant sera was developed using recombinant HIV proteins. Ten control infants, 19 uninfected infants of seropositive mothers and 12 HIV culture positive infants were studied at 3 month and 18 month time points. Prior to IgG depletion of the serum samples, 11/12 (92%) of the infected infants, 2/19 (11%) of the uninfected and none of the control infants were anti-HIV IgA positive at 3 months of age. After depletion, no anti-HIV IgA antibodies could be detected in the samples from uninfected infants, whereas the antibodies persisted in all 11 samples from the infected infants, resulting in sensitivity and specificity of 91.7% (95% confidence limits 59.8-99.6%) and 100% (79.1-100%) respectively. The assay might prove useful in the early diagnosis of HIV infection and can be performed at a fraction of the cost of commercially available western blot strips.

摘要

利用重组HIV蛋白开发了一种用于检测婴儿血清中抗HIV-1 IgA的简单斑点印迹筛查试验。在3个月和18个月的时间点对10名对照婴儿、19名血清反应阳性母亲的未感染婴儿以及12名HIV培养阳性婴儿进行了研究。在血清样本进行IgG去除之前，12名感染婴儿中有11名（92%）、19名未感染婴儿中有2名（11%），而对照婴儿中无一人在3个月大时抗HIV IgA呈阳性。去除后，未感染婴儿的样本中未检测到抗HIV IgA抗体，而感染婴儿的所有11个样本中抗体持续存在，敏感性和特异性分别为91.7%（95%置信区间59.8 - 99.6%）和100%（79.1 - 100%）。该检测方法可能在HIV感染的早期诊断中有用，并且可以以市售蛋白质印迹条成本的一小部分进行检测。