Willenbrock Klaus, Renné Christoph, Gaulard Philippe, Hansmann Martin-Leo
Senckenbergisches Institut für Pathologie, Klinikum der Johann Wolfgang Goethe Universität, Theodor Stern Kai 7, 60590 Frankfurt am Main, Germany.
Virchows Arch. 2005 Jan;446(1):15-20. doi: 10.1007/s00428-004-1114-1. Epub 2004 Oct 5.
The significance of T-cell proliferations in angioimmunoblastic lymphoma (AILD) is still enigmatic. Although classified as a malignant T-cell lymphoma in the World Health Organisation lymphoma classification, some cases of AILD lack dominant T-cell clones. In a previous study, based on single-cell polymerase chain reaction (PCR), we obtained similar results as studies of AILD using Southern blot or conventional PCR: some cases of AILD contained large T-cell clones, and, in other cases, T-cell clones were undetectable. As in single-cell studies, only a limited number of cells could be investigated; thus, we wanted to gain more insight into the amount and distribution of tumour cells. By applying triple immunofluorescent staining with antibodies directed against T-cell receptor Vbeta-family-specific epitopes, we investigated T-cell populations in AILD and their localisation in the tissue in relation to B cells (CD20) and follicular dendritic cells (CD21). In two of five cases investigated, only a minority of the T-cells compartment belonged to the tumour clone. Neoplastic T cells were found throughout the tissue, including areas dominated by B cells.
T细胞增殖在血管免疫母细胞性淋巴瘤（AILD）中的意义仍不明确。尽管在世界卫生组织淋巴瘤分类中被归类为恶性T细胞淋巴瘤，但一些AILD病例缺乏占主导地位的T细胞克隆。在先前的一项研究中，基于单细胞聚合酶链反应（PCR），我们获得了与使用Southern印迹法或传统PCR对AILD进行研究相似的结果：一些AILD病例含有大量T细胞克隆，而在其他病例中，T细胞克隆无法检测到。与单细胞研究一样，只能研究有限数量的细胞；因此，我们希望更深入地了解肿瘤细胞的数量和分布。通过应用针对T细胞受体Vβ家族特异性表位的抗体进行三重免疫荧光染色，我们研究了AILD中的T细胞群体及其在组织中相对于B细胞（CD20）和滤泡树突状细胞（CD21）的定位。在所研究的5例病例中的2例中，只有少数T细胞区室属于肿瘤克隆。在整个组织中都发现了肿瘤性T细胞，包括以B细胞为主的区域。
