Kim Myungshin, Kim Jayoung, Lim Jihyang, Kim Yonggoo, Han Kyungja, Kang Chang Suk
Department of Clinical Pathology, College of Medicine, The Catholic University of Korea, Seoul, Korea.
Ann Clin Lab Sci. 2004 Summer;34(3):307-13.
The automated analysis of bone marrow aspirates was performed to estimate the bone marrow cellularity and differential cell count. Total nucleated cell count (TNC) was measured using an automated hematology analyzer. TNC of the bone marrow correlated well with the bone marrow cellularity estimated by microscopic examination (r = 0.590, p <0.001). The bone marrow cellularity was readily confirmed as <30%, from 30 to 70%, or >70% according to TNC. Differential count of bone marrow cells was done with the combination of CD45 monoclonal antibody and propidium iodide using flow cytometry. Excellent correlations were obtained for the population distributions of normoblasts, eosinophils, lymphocytes, and blasts between the results of flow cytometry and manual differential counts. The percentage of mature granulocytes in flow cytometry showed good correlation with the manual percentage of metamyelocytes + neutrophils. The percentage of immature granulocytes by flow cytometry showed good correlation with the manual percentage of blasts + promyelocytes + myelocytes. These results demonstrate that analysis of bone marrow aspirates using an automated hematology analyzer is valuable in the determination of bone marrow cellularity. Moreover, as flow cytometry provides objective findings for percentages of major cell populations, it can serve as a method to automate bone marrow differentials.
对骨髓穿刺液进行自动分析，以评估骨髓细胞成分和细胞分类计数。使用自动血液分析仪测量有核细胞总数（TNC）。骨髓的TNC与通过显微镜检查估计的骨髓细胞成分密切相关（r = 0.590，p <0.001）。根据TNC，骨髓细胞成分很容易被确认为<30%、30%至70%或>70%。使用流式细胞术结合CD45单克隆抗体和碘化丙啶对骨髓细胞进行分类计数。流式细胞术结果与手工分类计数结果在正成红细胞、嗜酸性粒细胞、淋巴细胞和原始细胞的群体分布方面具有良好的相关性。流式细胞术中成熟粒细胞的百分比与手工计数中晚幼粒细胞+中性粒细胞的百分比具有良好的相关性。流式细胞术中未成熟粒细胞的百分比与手工计数中原始细胞+早幼粒细胞+中幼粒细胞的百分比具有良好的相关性。这些结果表明，使用自动血液分析仪分析骨髓穿刺液在确定骨髓细胞成分方面具有重要价值。此外，由于流式细胞术可为主要细胞群体的百分比提供客观结果，它可作为一种实现骨髓分类自动化的方法。
