Luo Jiankai, Redies Christoph
Institute of Anatomy I, Friedrich Schiller University, Teichgraben 7, D-07743 Jena, Germany.
J Neurosci Methods. 2004 Oct 30;139(2):241-5. doi: 10.1016/j.jneumeth.2004.04.032.
In cerebellar development, Purkinje cells transiently express various genes, whose expression is compartmentalized into different mediolaterial or parasagittal domains. The study of these genes will help us to understand the functional organization of the cerebellum. In the present work, we establish an efficient in vivo electroporation technique to transfer genes into Purkinje cells of the developing chicken cerebellum, as demonstrated by green fluorescent protein. This method can be applied to analyze gene function and regulation in cerebellar Purkinje cells.
在小脑发育过程中，浦肯野细胞会短暂表达各种基因，这些基因的表达在不同的内外侧或矢状旁区域呈分隔状态。对这些基因的研究将有助于我们了解小脑的功能组织。在本研究中，我们建立了一种高效的体内电穿孔技术，将基因导入发育中的鸡小脑浦肯野细胞，绿色荧光蛋白的表达证明了这一点。该方法可用于分析小脑浦肯野细胞中的基因功能和调控。
