分光光度法测定溶解于磷酸中的几丁质材料的脱乙酰度。

Spectrophotometric determination of deacetylation degree of chitinous materials dissolved in phosphoric acid.

作者信息

Hsiao Hsien-Yi, Tsai Chih-Cheng, Chen Suming, Hsieh Bo-Chuan, Chen Richie L C

机构信息

Biosensor Laboratory, Department of Bio-Industrial Mechatronics Engineering, College of Bioresources and Agriculture, National Taiwan University, 136 Chou-Shan Road, Taipei, Taiwan 106, R.O.C.

出版信息

Macromol Biosci. 2004 Oct 20;4(10):919-21. doi: 10.1002/mabi.200400084.

DOI:10.1002/mabi.200400084

PMID:15490434

Abstract

A simple spectrophotometric method is proposed for determining deacetylation degrees (DD) of chitinous materials using phosphoric acid as the UV-transparent solvent system. Calibrating by the extinction coefficients (A(210)) of D-glucosamine and N-acetyl-D-glucosamine, DD values (24-88%) were computed numerically. The results correlated well (R(2) = 0.9805, n = 50) with those obtained by solid-state (13)C NMR. Comparison of the results obtained by the proposed UV method and solid-state (13)C NMR.

摘要

提出了一种简单的分光光度法，以磷酸作为紫外透明溶剂体系来测定几丁质材料的脱乙酰度（DD）。通过D-葡萄糖胺和N-乙酰-D-葡萄糖胺的消光系数（A(210)）进行校准，以数值方式计算出DD值（24 - 88%）。结果与通过固态(13)C NMR获得的结果具有良好的相关性（R(2) = 0.9805，n = 50）。比较了所提出的紫外方法和固态(13)C NMR所获得的结果。