Wang Jen-You, Chao Yun-Peng
Department of Chemical Engineering, Feng Chia University, 100 Wenhwa Road, Taichung, Taiwan.
Appl Environ Microbiol. 2006 Jan;72(1):927-31. doi: 10.1128/AEM.72.1.927-931.2006.
To explore chitin-binding domain (ChBD)-based cell immobilization, a tripartite gene fusion consisting of an in-frame fusion of ChBD to lpp and ompA was constructed and expressed in Escherichia coli. ChBD-displayed cells exhibited highly specific and stable binding to chitin within a wide range of pHs (5 to 8) and temperatures (15 to 37 degrees C). These results illustrate the promising use of this approach for engineering applications.
为了探索基于几丁质结合域(ChBD)的细胞固定化方法,构建了一个由ChBD与lpp和ompA的读码框内融合组成的三方基因融合体,并在大肠杆菌中表达。展示ChBD的细胞在广泛的pH值范围(5至8)和温度范围(15至37摄氏度)内表现出与几丁质高度特异性且稳定的结合。这些结果表明这种方法在工程应用中具有广阔前景。