一个与SNF2同源的酿酒酵母必需基因在一个新家族中编码一种与解旋酶相关的蛋白质。
An essential Saccharomyces cerevisiae gene homologous to SNF2 encodes a helicase-related protein in a new family.
作者信息
Laurent B C, Yang X, Carlson M
机构信息
Department of Genetics and Development, Columbia University, College of Physicians and Surgeons, New York, New York 10032.
出版信息
Mol Cell Biol. 1992 Apr;12(4):1893-902. doi: 10.1128/mcb.12.4.1893-1902.1992.
The Saccharomyces cerevisiae SNF2 gene affects the expression of many diversely regulated genes and has been implicated in transcriptional activation. We report here the cloning and characterization of STH1, a gene that is homologous to SNF2. STH1 is essential for mitotic growth and is functionally distinct from SNF2. A bifunctional STH1-beta-galactosidase protein is located in the nucleus. The predicted 155,914-Da STH1 protein is 72% identical to SNF2 over 661 amino acids and 46% identical over another stretch of 66 amino acids. Both STH1 and SNF2 contain a putative nucleoside triphosphate-binding site and sequences resembling the consensus helicase motifs. The large region of homology shared by STH1 and SNF2 is conserved among other eukaryotic proteins, and STH1 and SNF2 appear to define a novel family of proteins related to helicases.
酿酒酵母SNF2基因影响许多受不同调控基因的表达，并与转录激活有关。我们在此报告STH1基因的克隆和特性，STH1基因与SNF2基因同源。STH1对有丝分裂生长至关重要，且在功能上与SNF2不同。一种双功能的STH1-β-半乳糖苷酶蛋白位于细胞核中。预测的155914道尔顿的STH1蛋白在661个氨基酸上与SNF2有72%的同一性，在另外66个氨基酸的区域有46%的同一性。STH1和SNF2都含有一个假定的核苷三磷酸结合位点以及类似于共有解旋酶基序的序列。STH1和SNF2共有的大片同源区域在其他真核生物蛋白中是保守的，并且STH1和SNF2似乎定义了一个与解旋酶相关的新型蛋白家族。
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