使用非共价酶-纳米颗粒共轭物实现单层控制的底物选择性。

Monolayer-controlled substrate selectivity using noncovalent enzyme-nanoparticle conjugates.

作者信息

Hong Rui, Emrick Todd, Rotello Vincent M

机构信息

Department of Chemistry, University of Massachusetts Amherst, Amherst, Massachusetts 01003, USA.

出版信息

J Am Chem Soc. 2004 Oct 27;126(42):13572-3. doi: 10.1021/ja0461163.

DOI:10.1021/ja0461163

PMID:15493887

Abstract

Electrostatic interactions were used to noncovalently conjugate chymotrypsin to gold nanoparticles featuring hybrid tetraethylene(glycol)alkanethiol monolayers terminated with carboxylate groups. This conjugation process greatly alters the substrate selectivity of the adsorbed chymotrypsin, inhibiting the hydrolysis of anionic subtrates without affecting the hydrolysis rate of cationic analogues.

摘要

利用静电相互作用将胰凝乳蛋白酶非共价连接到具有以羧基终止的混合四甘醇链烷硫醇单层的金纳米颗粒上。这种连接过程极大地改变了吸附的胰凝乳蛋白酶的底物选择性，抑制了阴离子底物的水解，而不影响阳离子类似物的水解速率。