Zheng Ming, Davidson Fredric, Huang Xueying
DuPont Central Research and Development, Experimental Station, Wilmington, Delaware 19880, USA.
J Am Chem Soc. 2003 Jul 2;125(26):7790-1. doi: 10.1021/ja0350278.
The usefulness of the hybrid materials of nanoparticles and biological molecules in many occasions depends on how well one can achieve a rational design based on specific binding and programmable assembly. Nonspecific binding between nanoparticles and biomolecules is one of the major barriers for achieving its utilities in a biological system. In this paper, we demonstrate a new approach to eliminate nonspecific interactions between nanoparticles and proteins by synthesizing ethylene glycol protected gold nanoparticles. We discovered that with the water content optimized in the range of 9-18% in the reaction mixture, di-, tri-, and tetra(ethylene glycol) protected gold nanoparticles Au-S-EGn (n = 2, 3, and 4) could be directly synthesized. These gold nanoparticles that are bonded with a uniform monolayer with defined length varying from 0.8 to 1.6 nm (from molecular modeling) have great stability in aqueous solutions with a high concentration of electrolyte and organic solutions. Using ion-exchange chromatography and gel electrophoresis, we demonstrated that these Au-S-EGn (n = 2, 3, or 4) nanoparticles have complete resistance to protein nonspecific interactions. These types of nanoparticles provide a fundamental starting material for designing hybrid materials composed of metallic nanoparticles and biomolecules.
纳米颗粒与生物分子的杂化材料在许多情况下的实用性取决于人们基于特异性结合和可编程组装实现合理设计的程度。纳米颗粒与生物分子之间的非特异性结合是在生物系统中实现其效用的主要障碍之一。在本文中，我们展示了一种通过合成乙二醇保护的金纳米颗粒来消除纳米颗粒与蛋白质之间非特异性相互作用的新方法。我们发现，当反应混合物中的水含量在9 - 18%范围内优化时，可以直接合成二、三、四（乙二醇）保护的金纳米颗粒Au - S - EGn（n = 2、3和4）。这些与长度从0.8到1.6纳米（通过分子模拟）不等的均匀单层结合的金纳米颗粒在高浓度电解质水溶液和有机溶液中具有很高的稳定性。使用离子交换色谱法和凝胶电泳，我们证明这些Au - S - EGn（n = 2、3或4）纳米颗粒对蛋白质非特异性相互作用具有完全抗性。这些类型的纳米颗粒为设计由金属纳米颗粒和生物分子组成的杂化材料提供了基本的起始材料。
