Jurczyk Agnieszka P, Gałecki Piotr, Kedziora Józef, Jankowska Beata, Meissner Ewa, Smigielski Janusz, Berent Jarosław, Szram Stefan
Z Katedry i Zakładu Medycyny Sadowej UM w Lodzi.
Arch Med Sadowej Kryminol. 2004 Apr-Sep;54(2-3):117-24.
In forensic medicine practice poisonings are rather frequent, and among them, those caused by fatal "substitution" of ethyl alcohol. One of the most frequently encountered "substitutes" for ethyl alcohol is methanol. The purpose of our research was to determine the concentration of malonic dialdehyde as the expression of lipid peroxidation and antioxidant enzyme activity after dosed chronic ethyl and methyl alcohol intoxication. The experiment was conducted on approx. 6 month-old male inbred Lewis rats each weighing approx. 250 g. Ethanol and methanol solution was given in the concentration 1.0 M. The control group of rats received water. Each experimental group numbered 30 rats, this number was divided into three sub-groups, which were put-down at 4, 8 and 12 weeks. The activity of superoxide dismutase (CuZu-SOD) was determined by the Misra-Fridovich method, catalase (CAT) by the Beers-Sizer method. The concentration of malonic dialdehyde (MDA) was determined using the method of Placer et al. by assessing the concentration of TBARS compounds. Results are expressed as a mean +/- SD. The paired Student's test for small groups were used. Superoxide dismutase SOD1 activity decreased compared with the control group throughout the duration of the experiment from 2212 U/gHb to 1676 U/gHb for ethanol and from 2212 U/gHb to 945 U/gHb for methanol. Catalase activity for methanol decreased from 9.1 U/gHb to 5.1 U/gHb, for ethanol to 7.4 U/gHb. In the 4th week of the experiment increase of malonyl dialdehyde concentration for methanol group was observed--from 0.14 umol/gHb to 0.34 umol/Hb; after 8th weeks it decreased to 0.2 umol/gHb and in the 12th week increased to 0.23 umol/gHb. For ethanol these changes was less visible and reached the level of 0.24 umol/l. The statistical processing of the results was performed on the basis of parametric tests (the t-Student test for small experiments) and computer software Statistica. The statistical significance was set for p<0.05.
在法医学实践中，中毒情况较为常见，其中由致命的乙醇“替代物”导致的中毒颇为典型。乙醇最常遇到的“替代物”之一是甲醇。我们研究的目的是确定在给予慢性乙醇和甲醇中毒剂量后，丙二醛浓度作为脂质过氧化的指标以及抗氧化酶活性。实验选用约6月龄、体重约250克的雄性近交系Lewis大鼠进行。给予浓度为1.0 M的乙醇和甲醇溶液。对照组大鼠给予水。每个实验组有30只大鼠，该数量分为三个亚组，分别在第4、8和12周处死。超氧化物歧化酶（铜锌超氧化物歧化酶，CuZu - SOD）活性采用米斯拉 - 弗里多维奇法测定，过氧化氢酶（CAT）活性采用比尔斯 - 西泽法测定。丙二醛（MDA）浓度采用普拉瑟等人的方法，通过评估硫代巴比妥酸反应物（TBARS）化合物的浓度来测定。结果以平均值±标准差表示。对小组采用配对学生检验。在整个实验过程中，超氧化物歧化酶SOD1活性与对照组相比有所下降，乙醇组从2212 U/gHb降至1676 U/gHb，甲醇组从2212 U/gHb降至945 U/gHb。甲醇组过氧化氢酶活性从9.1 U/gHb降至5.1 U/gHb，乙醇组降至7.4 U/gHb。在实验第4周，观察到甲醇组丙二酰二醛浓度升高，从0.14 μmol/gHb升至0.34 μmol/Hb；第8周后降至0.2 μmol/gHb，第12周又升至0.23 μmol/gHb。乙醇组的这些变化不太明显，达到0.24 μmol/l的水平。结果的统计处理基于参数检验（小实验的t检验）和计算机软件Statistica进行。设定统计学显著性为p < 0.05。
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