流式细胞术在血小板疾病中的应用。

Application of flow cytometry to platelet disorders.

作者信息

Linden Matthew D, Frelinger Andrew L, Barnard Marc R, Przyklenk Karin, Furman Mark I, Michelson Alan D

机构信息

Center for Platelet Function Studies, University of Massachusetts Medical School, Worcester, MA 01655, USA.

出版信息

Semin Thromb Hemost. 2004 Oct;30(5):501-11. doi: 10.1055/s-2004-835671.

DOI:10.1055/s-2004-835671

PMID:15497093

Abstract

Flow cytometry is a powerful and versatile tool that can be used to yield definitive information regarding the phenotypic status of platelets. The method provides a quantitative assessment of the physical and antigenic properties of platelets (e.g., surface expression of receptors, bound ligands, components of granules, or interactions of platelets with other platelets, other blood cells, or components of the plasma coagulation system), thereby facilitating the diagnosis of inherited or acquired platelet disorders (e.g., Bernard-Soulier syndrome, Glanzmann thrombasthenia, storage pool disease), the pathological activation of platelets (e.g., in the setting of acute coronary syndromes, cerebrovascular ischemia, peripheral vascular disease, cardiopulmonary bypass), and changes in the ability of platelets to activate via specific stimuli (e.g., efficacy of antiplatelet therapies). Accordingly, this review summarizes the key technical and methodologic components of flow cytometric analysis of platelets, as well as specific examples of its application to diagnosis and patient care.

摘要

流式细胞术是一种强大且通用的工具，可用于获取有关血小板表型状态的确切信息。该方法可对血小板的物理和抗原特性进行定量评估（例如，受体的表面表达、结合的配体、颗粒成分，或血小板与其他血小板、其他血细胞或血浆凝血系统成分的相互作用），从而有助于诊断遗传性或获得性血小板疾病（例如，伯纳德-索利尔综合征、血小板无力症、储存池病）、血小板的病理性激活（例如，在急性冠状动脉综合征、脑血管缺血、外周血管疾病、体外循环的情况下），以及血小板通过特定刺激激活能力的变化（例如，抗血小板治疗的疗效）。因此，本综述总结了血小板流式细胞术分析的关键技术和方法学组成部分，以及其在诊断和患者护理中的具体应用实例。