微囊藻毒素963A，一种微囊藻PCC 7806的胰凝乳蛋白酶抑制剂。

Cyanopeptolin 963A, a chymotrypsin inhibitor of Microcystis PCC 7806.

作者信息

Bister Bojan, Keller Simone, Baumann Heike I, Nicholson Graeme, Weist Stefan, Jung Günther, Süssmuth Roderich D, Jüttner Friedrich

机构信息

Limnological Station, Institute of Plant Biology, University of Zürich, Seestrasse 187, 8802 Kilchberg, Switzerland.

出版信息

J Nat Prod. 2004 Oct;67(10):1755-7. doi: 10.1021/np049828f.

DOI:10.1021/np049828f

PMID:15497957

Abstract

A new depsipeptide, cyanopeptolin 963 A (1), was isolated from an axenic strain of the toxic freshwater cyanobacterium Microcystis PCC 7806. The structure of this compound was elucidated by chemical and spectroscopic analyses, including high-resolution ESI-FTICR-MS, 2-D NMR, and GC-MS of the hydrolysate. The major structural difference compared to previously characterized cyanopeptolins of this strain is the replacement of the basic amino acid in position 3 by L-tyrosine. Compound 1 displayed inhibitory activity against chymotrypsin with an IC50 value of 0.9 microM.

摘要

从有毒淡水蓝藻微囊藻PCC 7806的无菌菌株中分离出一种新的缩肽，蓝藻肽963 A（1）。通过化学和光谱分析，包括高分辨率电喷雾傅里叶变换离子回旋共振质谱、二维核磁共振以及水解产物的气相色谱-质谱联用，阐明了该化合物的结构。与该菌株先前鉴定的蓝藻肽相比，主要的结构差异在于第3位的碱性氨基酸被L-酪氨酸取代。化合物1对胰凝乳蛋白酶表现出抑制活性，IC50值为0.9微摩尔。