Das Dipankar, Gares Sheryl L, Nagata Les P, Suresh Mavanur R
Faculty of Pharmacy and Pharmaceutical Sciences, University of Alberta, Edmonton, AB, Canada, T6G 2N8.
Antiviral Res. 2004 Nov;64(2):85-92. doi: 10.1016/j.antiviral.2004.06.008.
The E1 and E2 glycoproteins of Western Equine Encephalitis (WEE) are candidate antigens for WEE subunit vaccine development. We have cloned the E1 gene of WEE virus and expressed it in Escherichia coli as inclusion bodies. The inclusion bodies were successfully solubilised, refolded and the immunogenicity of this unglycosylated protein was assessed in mice. Immunization of mice with recombinant E1 protein generated both humoral and cell-mediated immune responses, indicating the recombinant E1 protein is immunogenic. Challenge of E1-immunized mice with live WEE virus demonstrated little or no protection from this E. coli-derived non-glycosylated subunit.
西部马脑炎（WEE）的E1和E2糖蛋白是WEE亚单位疫苗开发的候选抗原。我们克隆了WEE病毒的E1基因，并在大肠杆菌中作为包涵体进行表达。包涵体成功溶解、复性，并在小鼠中评估了这种未糖基化蛋白的免疫原性。用重组E1蛋白免疫小鼠产生了体液免疫和细胞介导的免疫反应，表明重组E1蛋白具有免疫原性。用活的WEE病毒攻击经E1免疫的小鼠，结果显示这种源自大肠杆菌的非糖基化亚单位几乎没有或根本没有保护作用。
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