Hiratsuka Masahiro, Ebisawa Aiko, Matsubara Yoichi, Kure Shigeo, Konno Yumiko, Sasaki Takamitsu, Mizugaki Michinao
Department of Clinical Pharmaceutics, Tohoku Pharmaceutical University, Sendai, Japan.
Drug Metab Pharmacokinet. 2004 Aug;19(4):303-7. doi: 10.2133/dmpk.19.303.
Using competitive allele-specific oligonucleotide hybridization with immunochromatographic strip (CASSOH), we have developed a simplified method for the detection of eight polymorphisms that are especially important in the identification of drug responders or non-responders and patients at increased risk of drug toxicity. The genotyping method is unambiguously determined by the presence or the absence of visible purple lines on the immunochromatographic strip, and results are obtained within 5 min after PCR. This method is rapid, highly sensitive, simplified, and should be suitable for point-of-care genotyping in clinical settings.
我们利用竞争性等位基因特异性寡核苷酸与免疫层析试纸条杂交技术（CASSOH），开发了一种简化方法，用于检测8种多态性，这些多态性在识别药物反应者或无反应者以及药物毒性风险增加的患者中尤为重要。通过免疫层析试纸上可见紫色条带的有无来明确确定基因分型方法，PCR后5分钟内即可获得结果。该方法快速、高度灵敏、简化，应适用于临床即时基因分型。
