基于糖基化金纳米粒子的即时诊断：从聚集到侧向流。

Glycosylated gold nanoparticles in point of care diagnostics: from aggregation to lateral flow.

机构信息

Department of Chemistry, University of Warwick, Gibbet Hill Road, CV4 7AL, Coventry, UK.

Oxford University Clinical Academic Graduate School, John Radcliffe Hospital Oxford, Oxford, OX3 9DU, UK.

出版信息

Chem Soc Rev. 2022 Aug 15;51(16):7238-7259. doi: 10.1039/d2cs00267a.

Current point-of-care lateral flow immunoassays, such as the home pregnancy test, rely on proteins as detection units ( antibodies) to sense for analytes. Glycans play a fundamental role in biological signalling and recognition events such as pathogen adhesion and hence they are promising future alternatives to antibody-based biosensing and diagnostics. Here we introduce the potential of glycans coupled to gold nanoparticles as recognition agents for lateral flow diagnostics. We first introduce the concept of lateral flow, including a case study of lateral flow use in the field compared to other diagnostic tools. We then introduce glycosylated materials, the affinity gains achieved by the cluster glycoside effect and the current use of these in aggregation based assays. Finally, the potential role of glycans in lateral flow are explained, and examples of their successful use given.

目前的即时侧向流动免疫分析，如家用妊娠测试，依赖蛋白质作为检测单元（抗体）来检测分析物。糖在生物信号和识别事件中起着至关重要的作用，如病原体黏附，因此它们是抗体为基础的生物传感和诊断的有前途的未来替代品。在这里，我们介绍了将糖与金纳米粒子偶联作为侧向流动诊断的识别剂的潜力。我们首先介绍了侧向流动的概念，包括侧向流动在现场应用的案例研究与其他诊断工具的比较。然后我们介绍了糖基化材料、糖基簇效应所获得的亲和力增益以及这些材料在聚集基测定中的当前应用。最后，解释了糖在侧向流动中的潜在作用，并给出了它们成功应用的例子。