Lilleväli Kersti, Matilainen Tanja, Karis Alar, Salminen Marjo
Institute of Biotechnology, University of Helsinki, Helsinki, Finland.
Dev Dyn. 2004 Dec;231(4):775-81. doi: 10.1002/dvdy.20185.
Gata2 and Gata3 belong to the Gata family of transcription factors in vertebrates that bind to a consensus "GATA" DNA sequence. The Gata3 gene is one of the earliest markers for the developing mouse inner ear. Ear morphogenesis is blocked in Gata3-deficient embryos, whereas nothing was known of the role of Gata2 in mouse inner ear. Here, we have compared the expression patterns of Gata2 and Gata3 during normal inner ear development and investigated their relationship in mice where either Gata3 or Gata2 has been inactivated. The expression of the two Gata genes is highly overlapping at embryonic day (E)10.5 but becomes increasingly distinct later. Whereas Gata2 is predominantly expressed in the dorsal vestibular system, Gata3 was detected mainly in the ventral cochlear duct and ganglion. No phenotypic abnormalities were observed in the inner ear of Gata2-/- embryos before lethality at E10.5 and Gata3 expression was unchanged. In contrast, a delay and strong reduction of Gata2 expression was detected in Gata3-/- otic epithelium.
Gata2和Gata3属于脊椎动物转录因子的Gata家族，它们与共有“GATA”DNA序列结合。Gata3基因是发育中的小鼠内耳最早的标志物之一。在Gata3缺陷型胚胎中，耳形态发生受阻，而Gata2在小鼠内耳中的作用尚不清楚。在这里，我们比较了Gata2和Gata3在正常内耳发育过程中的表达模式，并研究了在Gata3或Gata2失活的小鼠中它们之间的关系。这两个Gata基因在胚胎第（E）10.5天的表达高度重叠，但后来变得越来越不同。Gata2主要表达于背侧前庭系统，而Gata3主要在腹侧耳蜗管和神经节中检测到。在E10.5致死前，Gata2-/-胚胎的内耳未观察到表型异常，Gata3表达也未改变。相反，在Gata3-/-耳上皮中检测到Gata2表达延迟且显著降低。
