Hu Jian-Guo, Fu Sai-Li, Zhang Kai-Hua, Li Ying, Yin Lan, Lu Pei-Hua, Xu Xiao-Ming
Department of Neurobiology, Shanghai Second Medical University, Shanghai, Peoples Repubic of China.
J Neurosci Res. 2004 Dec 1;78(5):637-46. doi: 10.1002/jnr.20317.
The use of neural stem cells (NSCs) or their progeny oligodendrocyte precursor cells (OPCs) represents a promising repair strategy for many neurological disorders. However, the molecular events and biological features during the transition from NSCs to OPCs remain unclear. In the present study, we isolated NSCs from the embryonic rat forebrain and induced them into OPCs by using B104 conditioned medium (B104CM) in vitro. We then employed cDNA array technology to compare changes in gene expression between the two cell populations. Among 1,176 genes examined, 40 were differentially expressed, and some of them may be involved in OPC differentiation from NSCs. Our findings thus provide new insights into the molecular basis of differentiation of OPCs from NSCs.
使用神经干细胞（NSCs）或其后代少突胶质细胞前体细胞（OPCs）是治疗许多神经系统疾病的一种有前景的修复策略。然而，从神经干细胞向少突胶质细胞前体细胞转变过程中的分子事件和生物学特征仍不清楚。在本研究中，我们从胚胎大鼠前脑中分离出神经干细胞，并在体外使用B104条件培养基（B104CM）将其诱导为少突胶质细胞前体细胞。然后，我们采用cDNA阵列技术比较这两种细胞群体之间基因表达的变化。在所检测的1176个基因中，有40个基因差异表达，其中一些基因可能参与了少突胶质细胞前体细胞从神经干细胞的分化过程。因此，我们的研究结果为少突胶质细胞前体细胞从神经干细胞分化的分子基础提供了新的见解。
