Zhang Hui, Li Xiaoxin, Li Bin, Da Jiping
Department of Ophthalmology, Haidian Hospital, Beijing 100080, China.
Yan Ke Xue Bao. 2004 Sep;20(3):194-201.
To compare the histological alteration of retina with various spot intensities between diode and argon lasers in order to instruct the clinical use of 810 nm diode laser.
Transpupillary retinal photocoagulations were performed on 42 eyes of 27 pigmented rabbits. Histopathologic alteration of lesions in different intensities and different time intervals after irradiation produced by diode and argon laser was observed and compared using light microscopy. Areas of various lesions measured by image analysis system (CMIAS) were compared quantitatively.
Histopathologically, two-week-old grade 2 lesions produced by diode laser induced the disappearance of outer nuclear cells. More than a half of all showed reduction in number of outer nuclear layer cells in argon. Fibroblasts appeared in the diode grade 3 lesions 5 days after irradiation. CMIAS data showed that all the areas of diode lesions immediately after photocoagulation were to be larger than those of argon laser lesions in the same spot intensity (P < 0.05). However, twenty-four hours after photocoagulation, the area of the diode lesions increased less than that of the argon laser lesions (8% vs. 23%).
The acute histological effect caused by 810 nm diode laser and argon green laser is similar, while the expansion of lesion area 24 hours after photocoagulation was less with the diode laser compared to the argon. This may be the first report in the literature regarding quantitative analysis of the delayed reaction of argon green lasers.
比较二极管激光和氩激光在不同光斑强度下对视网膜的组织学改变，以指导810nm二极管激光的临床应用。
对27只色素兔的42只眼进行经瞳孔视网膜光凝。使用光学显微镜观察并比较二极管激光和氩激光照射后不同强度、不同时间间隔病变的组织病理学改变。用图像分析系统（CMIAS）对各种病变面积进行定量比较。
组织病理学上，二极管激光产生的两周龄2级病变导致外核层细胞消失。氩激光照射的病变中超过一半外核层细胞数量减少。二极管激光3级病变在照射后5天出现成纤维细胞。CMIAS数据显示，相同光斑强度下，光凝后即刻二极管激光病变的所有面积均大于氩激光病变（P<0.05）。然而，光凝24小时后，二极管激光病变面积的增加小于氩激光病变（8%对23%）。
810nm二极管激光和氩绿激光引起的急性组织学效应相似，但与氩激光相比，二极管激光光凝24小时后病变面积的扩大较小。这可能是文献中关于氩绿激光延迟反应定量分析的首次报道。
