Seibert Carla Simone, Oliveira Mara Regina L, Gonçalves Luís Roberto C, Santoro Marcelo Larami, Sano-Martins Ida Sigueko
Laboratório de Fisiopatologia, Instituto Butantan, Av. Vital Brazil, 1500, 05503-900 São Paulo, Brazil.
Toxicon. 2004 Dec 1;44(7):793-9. doi: 10.1016/j.toxicon.2004.08.019.
Hemostatic disturbances are frequent findings in human accidents caused by Lonomia obliqua caterpillars in the southern region of Brazil. In severe envenomation, patients may present life-threatening bleedings. Such disturbances may be mimicked in rats, which also develop intravascular hemolysis. The scope of this study was to investigate the time-course and intensity of intravascular hemolysis induced by i.d. injection of 750 microg/kg crude L. obliqua bristle extract in rats. Total blood cell count, reticulocyte count, plasma hemoglobin and haptoglobin assays were performed in control and envenomed rats at 1, 6, 24 and 48 h after envenomation. Rats presented a drastic drop of haptoglobin levels at 1 and 6h with increased plasma hemoglobin levels, a decrease in packed cell volume values at 6, 24 and 48 h, and increased reticulocyte counts throughout after envenomation. Such observations indicated that intravascular hemolysis occurred as early as 1h following envenomation, and lasted for more than 6h. Intravascular hemolysis is probably induced by phospholipase A(2) and other proteins with direct hemolytic activity present in crude caterpillar bristle extract.
在巴西南部地区，由斜纹天蛾毛虫导致的人类意外事件中，止血功能紊乱是常见的表现。在严重中毒时，患者可能会出现危及生命的出血情况。这种紊乱在大鼠中也可能出现，大鼠也会发生血管内溶血。本研究的目的是调查皮下注射750微克/千克斜纹天蛾毛虫刚毛粗提物诱导大鼠血管内溶血的时间进程和强度。在中毒后1、6、24和48小时，对对照大鼠和中毒大鼠进行全血细胞计数、网织红细胞计数、血浆血红蛋白和触珠蛋白检测。中毒后1小时和6小时，大鼠触珠蛋白水平急剧下降，血浆血红蛋白水平升高；在6小时、24小时和48小时，红细胞压积值降低；中毒后网织红细胞计数持续增加。这些观察结果表明，血管内溶血在中毒后1小时就已发生，且持续超过6小时。血管内溶血可能是由毛虫刚毛粗提物中存在的磷脂酶A₂和其他具有直接溶血活性的蛋白质诱导的。
