DL-α-硫辛酸对环磷酰胺诱导的大鼠睾丸氧化损伤的保护作用。
Protective effect of DL-alpha-lipoic acid in cyclophosphamide induced oxidative injury in rat testis.
作者信息
Selvakumar Elangovan, Prahalathan Chidambaram, Mythili Yenjerla, Varalakshmi Palaninathan
机构信息
Department of Medical Biochemistry, Dr. ALM Post Graduate Institute of Basic Medical Sciences, University of Madras, Taramani Campus, Chennai 600113, India.
出版信息
Reprod Toxicol. 2004 Dec;19(2):163-7. doi: 10.1016/j.reprotox.2004.06.015.
The aim of the present study was to evaluate the protective effect of DL-alpha-lipoic acid on the biochemical changes, tissue peroxidative damage and abnormal antioxidant levels in the rat testis during cyclophosphamide (CP)-induced injury. Adult male Wistar rats were divided into four treatment groups: (I) control, (II) 15 mg/kg CP once a week for 10 weeks by gavage, (III) 35 mg/kg lipoic acid once a week for 10 weeks by intraperitoneal injection, and (IV) CP plus lipoic acid (24 h prior to CP administration). Testicular toxicity, assessed by decreased enzymatic activities of lactate dehydrogenase and glucose-6-phosphate dehydrogenase, was reversed with lipoic acid pretreatment. CP-exposed rats (group II) showed abnormal levels of enzymes (superoxide dismutase, catalase, glutathione peroxidase, glutathione-S-transferase and glutathione reductase) and antioxidants (reduced glutathione, ascorbate and alpha-tocopherol) along with high malondialdehyde levels. In contrast, rats pretreated with lipoic acid (group IV) showed normal lipid peroxidation and antioxidant defenses. These findings indicate a cytoprotective role of lipoic acid in this experimental model of testicular toxicity.
本研究的目的是评估DL-α-硫辛酸对环磷酰胺（CP）诱导的大鼠睾丸损伤过程中生化变化、组织过氧化损伤及异常抗氧化水平的保护作用。成年雄性Wistar大鼠分为四个治疗组：（I）对照组；（II）每周一次经口灌胃给予15 mg/kg CP，共10周；（III）每周一次腹腔注射35 mg/kg硫辛酸，共10周；（IV）硫辛酸（在给予CP前24小时）加CP。通过乳酸脱氢酶和葡萄糖-6-磷酸脱氢酶的酶活性降低来评估的睾丸毒性，经硫辛酸预处理后得到逆转。CP处理的大鼠（II组）显示出酶（超氧化物歧化酶、过氧化氢酶、谷胱甘肽过氧化物酶、谷胱甘肽-S-转移酶和谷胱甘肽还原酶）和抗氧化剂（还原型谷胱甘肽、抗坏血酸和α-生育酚）水平异常，同时丙二醛水平升高。相比之下，硫辛酸预处理的大鼠（IV组）显示出正常的脂质过氧化和抗氧化防御。这些发现表明硫辛酸在该睾丸毒性实验模型中具有细胞保护作用。
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