Razak Redzuan Nul Hakim Abdul, Ismail Faridah, Isa Muhammad Lokman Md, Wahab Azantee Yazmie Abdul, Muhammad Hussin, Ramli Roszaman, Ismail Raja Arif Shah Raja
Department of Basic Medical Sciences, Kulliyyah of Allied Health Sciences, International Islamic University of Malaysia, Jalan Sultan Ahmad Shah, Bandar Indera Mahkota, 25200 Kuantan, Pahang, Malaysia.
Department of Basic Medical Sciences, Kulliyyah of Medicine, International Islamic University of Malaysia, Jalan Sultan Ahmad Shah, Bandar Indera Mahkota, 25200 Kuantan, Pahang, Malaysia.
Malays J Med Sci. 2019 Jan;26(1):44-57. doi: 10.21315/mjms2019.26.1.4. Epub 2019 Feb 28.
Cyclophosphamide (CP) is a widely used anti-neoplastic and immunosuppressive agent that is associated with adverse side effects including reproductive toxicity. (AM) is a traditional medicinal plant which was reported to exhibit high anti-oxidant and free radical scavenging properties. The present study was aimed to evaluate the protective effects of AM leaves extract on sperm quality following toxic exposure to CP.
Forty-eight male Sprague Dawley rats were allocated into eight groups of six rats ( = 6): control, CP only (200 mg kg), AM only (100 mg kg, 300 mg kg and 500 mg kg) and CP + AM (100 mg kg, 300 mg kg and 500 mg kg). Animals were sacrificed after 63 days of treatment and the sperm from the caudal epididymis was taken for sperm analysis.
The body and the reproductive organs weight, sperm count and motility did not differ between CP and other groups ( > 0.05). A significant increase ( < 0.05) in percentage of the dead and abnormal sperm were seen in the CP alone treated group compared to the control group. Co-administration of AM to the CP exposed rats significantly reduced the ( < 0.05) percentage of abnormal sperm as compared to the CP only group.
Overall, the present results represent the potential of AM to protect against CP induced reproductive toxicity.
环磷酰胺（CP）是一种广泛使用的抗肿瘤和免疫抑制剂，会产生包括生殖毒性在内的不良副作用。穿心莲（AM）是一种传统药用植物，据报道具有高抗氧化和自由基清除特性。本研究旨在评估穿心莲叶提取物对CP毒性暴露后精子质量的保护作用。
将48只雄性Sprague Dawley大鼠分为8组，每组6只（n = 6）：对照组、仅CP组（200 mg/kg）、仅AM组（100 mg/kg、300 mg/kg和500 mg/kg）以及CP + AM组（100 mg/kg、300 mg/kg和500 mg/kg）。治疗63天后处死动物，取附睾尾部精子进行精子分析。
CP组与其他组之间的体重、生殖器官重量、精子计数和活力无差异（P > 0.05）。与对照组相比，仅CP治疗组的死精子和异常精子百分比显著增加（P < 0.05）。与仅CP组相比，给CP暴露大鼠联合使用AM可显著降低（P < 0.05）异常精子百分比。
总体而言，本研究结果表明穿心莲具有预防CP诱导的生殖毒性的潜力。
