Oskam Irma C, Ropstad Erik, Smith Adrian J, Skaare Janneche U, Tverdal Aage, Berg Kjell Andersen, Wiger Richard
Department of Production Animal Clinical Science, Norwegian School of Veterinary Science, P.O. Box 8146, NO033 Oslo, Norway.
Reprod Toxicol. 2004 Dec;19(2):169-80. doi: 10.1016/j.reprotox.2004.07.005.
This study examined the effects of acute exposure to PCB99 (2,2',4,4',5-pentachlorobiphenyl), and PCB153 (2,2',4,4'5,5'-hexachlorobiphenyl), on spermatogenesis in 8-week-old C57BL6 mice. The mice were randomly allocated to PCB99 and PCB153 and a single dose of respectively 10 and 100 mg/kg was given by oral gavage. During the 6-week experiment, six mice per treatment group were sacrificed weekly, body weights were recorded and samples with respect to the male reproductive system were collected until further analysis. None of the treatments, showed changes in body weight or reproductive endpoints. Flow cytometric analysis revealed spermatogenesis to be unaffected. However, PCB99 and PCB153 showed a significant increase in Leydig cell apoptosis. The results from the present study indicate that the male reproductive system is relatively refractory to PCB99 and PCB153 at levels exceeding those of wildlife and humans, when exposed during adult life. However, the finding of apoptotic Leydig cells merits further investigation.
本研究检测了急性暴露于多氯联苯99（2,2',4,4',5-五氯联苯）和多氯联苯153（2,2',4,4',5,5'-六氯联苯）对8周龄C57BL6小鼠精子发生的影响。将小鼠随机分为多氯联苯99组和多氯联苯153组，分别经口灌胃给予10和100 mg/kg的单剂量。在为期6周的实验期间，每周处死每个治疗组的6只小鼠，记录体重，并采集雄性生殖系统样本直至进一步分析。所有处理均未显示体重或生殖终点有变化。流式细胞术分析显示精子发生未受影响。然而，多氯联苯99和多氯联苯153显示睾丸间质细胞凋亡显著增加。本研究结果表明，成年期暴露时，雄性生殖系统对超过野生动物和人类水平的多氯联苯99和多氯联苯153相对不敏感。然而，睾丸间质细胞凋亡的发现值得进一步研究。
