Kürner Julia, Medalia Ohad, Linaroudis Alexandros A, Baumeister Wolfgang
Department of Structural Biology, Max Planck Institute of Biochemistry, D-82152 Martinsried, Germany.
Exp Cell Res. 2004 Nov 15;301(1):38-42. doi: 10.1016/j.yexcr.2004.08.005.
Cryo-electron tomography (cryo-ET) is an emerging imaging technology that combines the potential of three-dimensional (3-D) imaging at molecular resolution (<5 nm) with a close-to-life preservation of the specimen. In conjunction with pattern recognition techniques, it enables us to map the molecular landscape inside cells. The application of cryo-ET to intact cells provides novel insights into the structure and the spatial organization of the cytoskeleton in prokaryotic and eukaryotic cells.
冷冻电子断层扫描（cryo-ET）是一种新兴的成像技术，它将分子分辨率（<5纳米）下的三维（3-D）成像潜力与对标本近乎真实的保存相结合。结合模式识别技术，它使我们能够绘制细胞内的分子图谱。将cryo-ET应用于完整细胞，为原核细胞和真核细胞中细胞骨架的结构和空间组织提供了新的见解。
