新生牛血清对人胎儿海马神经干细胞分化的影响
[Effects of neonatal calf serum on differentiation of human fetal neural stem cells in the hippocampus].
作者信息
Yin Xiao-juan, Feng Zhi-chun, Du Jiang
机构信息
Department of Pediatrics, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China.
出版信息
Di Yi Jun Yi Da Xue Xue Bao. 2004 Oct;24(10):1164-6, 1170.
OBJECTIVE
To investigate the effects of neonatal calf serum on the differentiation of human neural stem cells in the hippocampus.
METHODS
The effects of various concentrations of neonatal calf serum on the differentiation of human fetal neural stem cells in the hippocampus were observed in cell culture experiment and immunocytochemical staining.
RESULTS
Neonatal calf serum efficiently induced the differentiation of human fetal neural stem cells into neurons and astrocytes, whose amount varied between serum-free cell culture and the culture with neonatal calf serum of different concentrations. As the concentration of neonatal calf serum increased, the differentiation of human fetal neural stem cells shifted toward astrocytes, while the differentiation into neurons was decreased.
CONCLUSION
Neonatal calf serum causes alteration of the ratio between neurons and glial cells differentiated from human neural stem cells in the hippocampus.
目的
研究新生牛血清对海马区人神经干细胞分化的影响。
方法
通过细胞培养实验和免疫细胞化学染色，观察不同浓度新生牛血清对海马区人胎儿神经干细胞分化的影响。
结果
新生牛血清能有效诱导人胎儿神经干细胞分化为神经元和星形胶质细胞，无血清细胞培养与不同浓度新生牛血清培养时，二者数量有所不同。随着新生牛血清浓度升高，人胎儿神经干细胞的分化向星形胶质细胞偏移，而向神经元的分化减少。
结论
新生牛血清可导致海马区人神经干细胞分化产生的神经元与神经胶质细胞比例发生改变。
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