Patil B L, Rajasubramaniam S, Bagchi C, Dasgupta I
Department of Plant Molecular Biology, University of Delhi South Campus, Benito Juarez Road, New Delhi 11002, India.
Arch Virol. 2005 Feb;150(2):389-97. doi: 10.1007/s00705-004-0399-3. Epub 2004 Oct 20.
The biodiversity of geminiviruses associated with the Cassava Mosaic Disease (CMD) in India was investigated using PCR to specifically amplify the DNA of Indian cassava mosaic virus (ICMV) or Sri Lankan cassava mosaic virus (SLCMV) and also by using PCR to amplify specific viral genes, followed by digestion with different restriction endonucleases to obtain polymorphic patterns (PCR-RFLP). Results showed that both ICMV and SLCMV were present in mosaic-affected cassava; ICMV was geographically restricted to certain regions, whereas SLCMV was widespread. PCR-RFLP analysis showed that, in addition to ICMV-type and SLCMV-type patterns, a high proportion (40%) of the samples displayed novel patterns, some of which were localized in certain areas, whereas others were widely distributed.
利用聚合酶链式反应（PCR）特异性扩增印度木薯花叶病毒（ICMV）或斯里兰卡木薯花叶病毒（SLCMV）的DNA，并通过PCR扩增特定病毒基因，随后用不同的限制性内切酶进行酶切以获得多态性模式（PCR-RFLP），对印度与木薯花叶病（CMD）相关的双生病毒的生物多样性进行了研究。结果表明，ICMV和SLCMV均存在于受花叶病影响的木薯中；ICMV在地理上局限于某些地区，而SLCMV分布广泛。PCR-RFLP分析表明，除了ICMV型和SLCMV型模式外，高比例（40%）的样本呈现出新颖的模式，其中一些局限于某些区域，而另一些则广泛分布。
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