Borah Basanta Kumar, Dasgupta Indranil
Department of Plant Molecular Biology, University of Delhi South Campus, Benito Juarez Road, Delhi 110021, India.
Virus Genes. 2012 Oct;45(2):327-32. doi: 10.1007/s11262-012-0770-5. Epub 2012 Jun 14.
Cassava mosaic disease (CMD) is caused in India by two bipartite begomoviruses, Indian cassava mosaic virus (ICMV), and Sri Lankan cassava mosaic virus (SLCMV). Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was used as a rapid means of investigating the molecular diversity of ICMV and SLCMV in 38 samples of CMD-affected cassava plants under field conditions in new areas of cassava cultivation, along with traditional areas in southern India. A very large proportion of the samples showed SLCMV, based on a discriminatory PCR between SLCMV and ICMV, reported earlier. PCR-RFLP analysis of three regions of viral DNA indicated that in most samples, although the AC1 and the AV1 resembled SLCMV, as expected, the intergenic regions (binding site for host replication machinery) resembled ICMV more closely, indicating recombination events between ICMV and SLCMV. Results also indicate that the AC1 is more conserved within SLCMV compared to the AV1 gene.
木薯花叶病（CMD）在印度由两种双分体菜豆金色花叶病毒引起，即印度木薯花叶病毒（ICMV）和斯里兰卡木薯花叶病毒（SLCMV）。在印度南部的传统种植区以及木薯种植新区域的田间条件下，利用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）作为一种快速手段，对38份受CMD影响的木薯植株样本中的ICMV和SLCMV分子多样性进行了研究。根据之前报道的SLCMV和ICMV之间的鉴别性PCR，很大比例的样本显示感染了SLCMV。对病毒DNA三个区域的PCR-RFLP分析表明，在大多数样本中，尽管如预期的那样AC1和AV1与SLCMV相似，但基因间区域（宿主复制机制的结合位点）与ICMV更为相似，这表明ICMV和SLCMV之间发生了重组事件。结果还表明，与AV1基因相比，AC1在SLCMV内更为保守。
