编码人1型血管紧张素II受体的cDNA的分子克隆、序列分析及表达
Molecular cloning, sequence analysis and expression of a cDNA encoding human type-1 angiotensin II receptor.
作者信息
Takayanagi R, Ohnaka K, Sakai Y, Nakao R, Yanase T, Haji M, Inagami T, Furuta H, Gou D F, Nakamuta M
机构信息
Third Department of Internal Medicine, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
出版信息
Biochem Biophys Res Commun. 1992 Mar 16;183(2):910-6. doi: 10.1016/0006-291x(92)90570-b.
We isolated a cDNA encoding type-1 angiotensin II receptor from a human liver cDNA library. The cDNA had an open reading frame encoding a protein of 359 amino acid residues with a relative Mr of 41,060. The deduced amino acid sequence of the human angiotensin II (Ang II) receptor was 95.3% and 94.2% identical to those of bovine and rat type-1 Ang II receptors, respectively, and had a significant similarity with the G protein-coupled receptor. The rank order of the binding to the receptor expressed in COS-7 cells was Ang II greater than Ang III greater than Ang I. The expression of the Ang II receptor mRNA was detected in human liver, lung, adrenal and adrenocortical adenomas but not in adrenomedullary tumor, pheochromocytoma, by Northern blot analysis.
我们从人肝脏cDNA文库中分离出一种编码1型血管紧张素II受体的cDNA。该cDNA具有一个开放阅读框，编码一个由359个氨基酸残基组成的蛋白质，相对分子质量为41,060。推导的人血管紧张素II（Ang II）受体氨基酸序列与牛和大鼠1型Ang II受体的氨基酸序列分别有95.3%和94.2%的同一性，并且与G蛋白偶联受体有显著的相似性。在COS-7细胞中表达的受体上的结合活性顺序为：Ang II大于Ang III大于Ang I。通过Northern印迹分析，在人肝脏、肺、肾上腺和肾上腺皮质腺瘤中检测到了Ang II受体mRNA的表达，但在肾上腺髓质肿瘤、嗜铬细胞瘤中未检测到。
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