血浆内皮微粒升高：子痫前期与妊娠高血压的比较。

Elevated plasma endothelial microparticles: preeclampsia versus gestational hypertension.

作者信息

González-Quintero Víctor H, Smarkusky Loren P, Jiménez Joaquín J, Mauro Lucía M, Jy Wenche, Hortsman Laurence L, O'Sullivan Mary J, Ahn Yeon S

机构信息

Division of Maternal-Fetal Medicine, Department of Gynecology and Obstetrics, University of Miami School of Medicine, PO Box 016960 (R-136), Miami, FL 33101, USA.

出版信息

Am J Obstet Gynecol. 2004 Oct;191(4):1418-24. doi: 10.1016/j.ajog.2004.06.044.

DOI:10.1016/j.ajog.2004.06.044

PMID:15507976

Abstract

OBJECTIVE

Elevated plasma endothelial microparticle levels have been found to be elevated in women with preeclampsia. However, their role in distinguishing preeclampsia from gestational hypertension remains to be elucidated. The objectives of this study were to compare endothelial microparticle levels among patients with preeclampsia, gestational hypertension, and healthy pregnant control subjects and to evaluate the effect of plasma from women with preeclampsia and gestational hypertension on the release of endothelial microparticles by renal microvascular endothelial cells.

STUDY DESIGN

A prospective study was conducted on 52 women with preeclampsia, 20 women with gestational hypertension, and 38 healthy pregnant control subjects. Endothelial microparticles were measured by flow cytometry with fluorescent monoclonal mouse anti-human antibodies against CD31, CD42b, and CD62E.

RESULTS

CD31 + /42b - endothelial microparticle levels were 10497 +/- 5145 counts/microL in women with preeclampsia versus 6768 +/- 1810 counts/microL in women with gestational hypertension ( P < .01). In control subjects, CD31 + /42b - endothelial microparticle levels were 6119 +/- 3592 counts/microL. CD62E + endothelial microparticle levels were 1930 +/- 966 counts/microL in women with preeclampsia versus 822 +/- 150 counts/microL in women with gestational hypertension ( P <.01). In control subjects, CD62E + endothelial microparticle levels were 712 +/- 160 counts/microL. Incubation of renal microvascular endothelial cells with plasma from women with preeclampsia resulted in a rise in CD31 + and CD62E + endothelial microparticle levels as compared with women with gestational hypertension and control subjects.

CONCLUSION

Endothelial microparticle levels are higher in women with preeclampsia than in women with gestational hypertension and control subjects. The measurement of endothelial microparticles may be useful as a diagnostic tool for preeclampsia in pregnant women.

摘要

目的

子痫前期女性的血浆内皮微粒水平已被发现升高。然而，它们在子痫前期与妊娠高血压的鉴别诊断中的作用仍有待阐明。本研究的目的是比较子痫前期患者、妊娠高血压患者和健康妊娠对照者的内皮微粒水平，并评估子痫前期和妊娠高血压女性的血浆对肾微血管内皮细胞释放内皮微粒的影响。

研究设计

对52例子痫前期女性、20例妊娠高血压女性和38例健康妊娠对照者进行了一项前瞻性研究。采用流式细胞术，用荧光单克隆小鼠抗人抗体检测针对CD31、CD42b和CD62E的内皮微粒。

结果

子痫前期女性的CD31 + /42b - 内皮微粒水平为10497±5145个/微升，而妊娠高血压女性为6768±1810个/微升（P <.01）。在对照者中，CD31 + /42b - 内皮微粒水平为6119±3592个/微升。子痫前期女性的CD62E + 内皮微粒水平为1930±966个/微升，而妊娠高血压女性为822±150个/微升（P <.01）。在对照者中，CD62E + 内皮微粒水平为712±160个/微升。与妊娠高血压女性和对照者相比，用子痫前期女性的血浆孵育肾微血管内皮细胞导致CD31 + 和CD62E + 内皮微粒水平升高。