固氮细菌巴西伯克霍尔德氏菌产生一种新型的含yersiniose A的O-多糖。
Nitrogen-fixing bacterium Burkholderia brasiliensis produces a novel yersiniose A-containing O-polysaccharide.
作者信息
Mattos Katherine A, Todeschini Adriane R, Heise Norton, Jones Christopher, Previato Jose O, Mendonça-Previato Lucia
机构信息
Instituto de Biofísica Carlos Chagas Filho, Centro de Ciências da Saúde, Bloco G, Universidade Federal do Rio de Janeiro, 21944-970, Brazil.
出版信息
Glycobiology. 2005 Mar;15(3):313-21. doi: 10.1093/glycob/cwi009. Epub 2004 Oct 27.
Burkholderia brasiliensis, a Gram-negative diazotrophic endophytic bacterium, was first isolated from roots, stems, and leaves of rice plant in Brazil. The polysaccharide moiety was released by ammonolysis from the B. brasiliensis lipopolysaccharide (LPS), allowing the unambiguous characterization of a 3,6-dideoxy-4-C-(1-hydroxyethyl)-D-xylo-hexose (yersiniose A), an uncommon feature for Burkholderia LPS. The complete structure of the yersiniose A-containing O-antigen was identified by sugar and methylation analyses and NMR spectroscopy. Our results show that the repeating oligosaccharide motif of LPS O-chain consists of a branched tetrasaccharide with the following structure:-->2-alpha-d-Rhap-(1-->3)-[alpha-YerAp-(1-->2)]-alpha-D-Rhap-(1-->3)-alpha-D-Rhap-(1-->.
巴西伯克霍尔德菌是一种革兰氏阴性固氮内生细菌，最初从巴西水稻植株的根、茎和叶中分离得到。通过氨解从巴西伯克霍尔德菌脂多糖（LPS）中释放出多糖部分，从而明确鉴定出一种3,6-二脱氧-4-C-（1-羟乙基）-D-木糖己糖（yersiniose A），这是伯克霍尔德菌LPS的一个不常见特征。通过糖和甲基化分析以及核磁共振光谱确定了含yersiniose A的O抗原的完整结构。我们的结果表明，LPS O链的重复寡糖基序由具有以下结构的分支四糖组成：-->2-α-D-鼠李糖基-（1→3）-[α-YerAp-（1→2）]-α-D-鼠李糖基-（1→3）-α-D-鼠李糖基-（1→ 。
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