Kondakova Anna N, Bystrova Olga V, Shaikhutdinova Rima Z, Ivanov Sergey A, Dentovskaya Svetlana V, Shashkov Alexander S, Knirel Yuriy A, Anisimov Andrey P
N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russian Federation.
Carbohydr Res. 2009 Mar 10;344(4):531-4. doi: 10.1016/j.carres.2008.12.010. Epub 2008 Dec 16.
The O-specific polysaccharide was isolated by mild acid degradation of the lipopolysaccharide of Yersinia pseudotuberculosis O:4a and studied by NMR spectroscopy, including 2D ROESY and (1)H, (13)C HMBC experiments. The following structure of the pentasaccharide repeating unit of the polysaccharide was established, which differs from the structure reported earlier [Gorshkova, R. P. et al., Bioorg. Khim. 1983, 9, 1401-1407] in the linkage modes between the monosaccharides: [carbohydrate sequence see in text] where Tyv stands for 3,6-dideoxy-D-arabino-hexose (tyvelose). The structure of the Y. pseudotuberculosis O:4a antigen resembles that of Y. pseudotuberculosis O:2c, which differs in the presence of abequose (3,6-dideoxy-D-xylo-hexose) in place of tyvelose only.
通过对假结核耶尔森氏菌O:4a脂多糖进行温和酸降解，分离出O-特异性多糖，并通过核磁共振光谱进行研究，包括二维旋转核Overhauser效应光谱（2D ROESY）和氢-碳异核多键相关实验（(1)H,(13)C HMBC）。确定了该多糖五糖重复单元的以下结构，该结构在单糖之间的连接方式上与先前报道的结构[戈尔什科娃，R.P.等人，《生物有机化学》，1983年，9卷，1401 - 1407页]不同：[碳水化合物序列见正文]，其中Tyv代表3,6-二脱氧-D-阿拉伯己糖（泰威糖）。假结核耶尔森氏菌O:4a抗原的结构类似于假结核耶尔森氏菌O:2c的结构，二者的区别仅在于O:2c存在阿比可糖（3,6-二脱氧-D-木糖己糖）而不是泰威糖。
