Instituto de Biofísica Carlos Chagas Filho, Centro de Ciências da Saúde, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil.
Glycobiology. 2010 Mar;20(3):338-47. doi: 10.1093/glycob/cwp181. Epub 2009 Nov 18.
Burkholderia kururiensis, strain M130, an endophytic diazotrophic bacterium isolated from rice roots, produces acetylated acidic exopolysaccharides which can be separated by anion exchange chromatography. These were characterized by nuclear magnetic resonance spectroscopy, methylation analysis and Smith degradation. The exopolysaccharides eluted with 0.5 M NaCl were produced when the bacterium was grown in a medium containing mannitol as the sole carbon source, and showed to be a mixture of two different polymers, composed of hepta or octasaccharide repeat units, consistent with following structure: [structure: see text]. The ability of diazotrophic Burkholderia to produce two exopolysaccharides that differ by the presence of a terminal glucosyl residue provides insight into polysaccharide function with potentially significant biological consequences in the endophytic-host plant interaction.
库默里氏伯克霍尔德菌 M130 菌株，一种内生固氮菌，从水稻根部分离得到，产生乙酰化酸性胞外多糖，可以通过阴离子交换色谱分离。这些通过核磁共振波谱、甲基化分析和 Smith 降解进行了表征。当细菌在含有甘露醇作为唯一碳源的培养基中生长时，用 0.5 M NaCl 洗脱的胞外多糖被产生，并且显示为两种不同聚合物的混合物，由七或八糖重复单元组成，与以下结构一致：[结构：见文本]。具有固氮能力的伯克霍尔德氏菌能够产生两种胞外多糖，其区别在于末端葡萄糖基残基的存在，这为多糖功能提供了深入了解，这可能对内生-宿主植物相互作用具有重要的生物学意义。
