Oji Yusuke, Yano Masahiko, Nakano Yoko, Abeno Sakie, Nakatsuka Shin-Ichi, Ikeba Ai, Yasuda Takushi, Fujiwara Yoshiyuki, Takiguchi Shuji, Yamamoto Hirofumi, Fujita Seiichiro, Kanato Keisuke, Ito Ken, Jomgeow Tanyarat, Kawakami Manabu, Tsuboi Akihiro, Shirakata Toshiaki, Nishida Sumiyuki, Hosen Naoki, Oka Yoshihiro, Aozasa Katsuyuki, Monden Morito, Sugiyama Haruo
Department of Functional Diagnostic Science, Osaka University Graduate School of Medicine, 1-7, Yamada-oka, Suita, Osaka 565-0871, Japan.
Anticancer Res. 2004 Sep-Oct;24(5B):3103-8.
The Wilms' tumor gene WT1 is overexpressed in various kinds of solid cancers. However, it remains unclear whether WT1 is expressed in esophageal squamous cell carcinoma.
Expression of the WT1 gene was examined by real-time RT-PCR in 12 esophageal squamous cell carcinoma (ESCC) and by immunohistochemistry in 9 of these 12 and another 29.
Real-time RT-PCR showed that the WT1 mRNA was overexpressed in all of the 12 ESCC examined Immunohistochemical analysis showed that the WT1 protein was overexpressed in ESCC cells in 36 (95%) of the 38 examined Furthermore, expression of the WT1 protein was examined in 20 esophageal squamous dysplasia. The WT1 protein was overexpressed in 5 (45%) out of 11 mild dysplasia and in 8 (89%) out of 9 moderate to severe dysplasia.
These results may indicate an important role of the WT1 gene in the tumorigenesis of ESCC.
威尔姆斯瘤基因WT1在多种实体癌中过表达。然而，WT1在食管鳞状细胞癌中是否表达仍不清楚。
通过实时逆转录聚合酶链反应检测12例食管鳞状细胞癌中WT1基因的表达，并对其中9例以及另外29例进行免疫组织化学检测。
实时逆转录聚合酶链反应显示，在检测的所有12例食管鳞状细胞癌中WT1信使核糖核酸均过表达。免疫组织化学分析显示，在检测的38例中，36例（95%）食管鳞状细胞癌细胞中WT1蛋白过表达。此外，对20例食管鳞状发育异常进行了WT1蛋白表达检测。在11例轻度发育异常中的5例（45%）以及9例中重度发育异常中的8例（89%）中WT1蛋白过表达。
这些结果可能表明WT1基因在食管鳞状细胞癌的肿瘤发生中起重要作用。
