Reid S M, Paton D J, Wilsden G, Hutchings G H, King D P, Ferris N P, Alexandersen S
Institute for Animal Health, Pirbright Laboratory, Ash Road, Pirbright, Woking, Surrey GU24 0NF, UK.
J Comp Pathol. 2004 Nov;131(4):308-17. doi: 10.1016/j.jcpa.2004.05.003.
Automated real-time RT-PCR was evaluated as a diagnostic tool for swine vesicular disease virus (SVDV) infection on a range of samples (vesicular epithelium, serum, nasal swabs, faeces) from four inoculated and three in-contact pigs over a period of 28 days. Traditional diagnostic procedures (virus isolation, and ELISAs for antigen and antibody) were used in parallel. Each inoculated pig developed a significant viraemia and clinical disease, and excreted virus, which was transmitted to the in-contact animals. The latter, however, developed only a short-lived, low-level viraemia and no clinical disease. The RT-PCR and virus isolation were generally comparable in detecting SVDV in the serum and nasal swabs from inoculated and in-contact pigs up to day 6 after infection; it was possible, however, to isolate virus for a longer period from the faeces of a few pigs. This suggested that further optimization of the template extraction method was required to counteract the effects of RT-PCR inhibitors in faeces. It was concluded that the automated real-time RT-PCR is a useful diagnostic method for SVD in clinically or subclinically affected pigs and contributed to the study of the pathogenesis of SVD in the pigs.
在28天的时间里，对4头接种猪和3头接触猪的一系列样本（水疱上皮、血清、鼻拭子、粪便）进行了评估，以确定自动化实时逆转录聚合酶链反应（RT-PCR）作为猪水疱病病毒（SVDV）感染诊断工具的效果。同时使用了传统诊断程序（病毒分离以及抗原和抗体酶联免疫吸附测定）。每头接种猪都出现了显著的病毒血症和临床疾病，并排出病毒，病毒传播给了接触猪。然而，后者仅出现了短暂的、低水平的病毒血症，没有临床疾病。在感染后第6天之前，RT-PCR和病毒分离在检测接种猪和接触猪的血清及鼻拭子中的SVDV方面总体相当；不过，从少数猪的粪便中分离病毒的时间更长。这表明需要进一步优化模板提取方法，以抵消粪便中RT-PCR抑制剂的影响。得出的结论是，自动化实时RT-PCR是临床或亚临床感染猪SVD的一种有用诊断方法，有助于研究猪SVD的发病机制。
