实时逆转录聚合酶链反应在猪水疱病病毒检测中的应用。
Application of real-time reverse transcription polymerase chain reaction for the detection of SVDV.
作者信息
Niedbalski W
机构信息
Department of Foot-and-Mouth Disease, National Veterinary Research Institute, 98-220 Zduńska Wola, Poland.
出版信息
Pol J Vet Sci. 2009;12(1):119-21.
Application of real-time RT-PCR (rRT-PCR) for detection of swine vesicular disease virus (SVDV) in samples of archival SVDV isolates and clinical samples collected from SVDV infected pigs was described. A primer set that targets the IRES region of the SVDV genome and TaqMan probe specific for a highly conserved region in SVDV RNA IRES region were used. The assay detected viral RNA in all tested archival strains of SVDV isolated in Europe during years 1972-73 and 1992 as well as in clinical samples collected from experimentally infected pigs. The rRT-PCR can provide quantitative and qualitative information and is more sensitive and faster to perform than the conventional RT-PCR.
描述了实时逆转录聚合酶链反应（rRT-PCR）在检测猪水疱病病毒（SVDV）存档分离株样本以及从感染SVDV的猪采集的临床样本中的应用。使用了一组靶向SVDV基因组内部核糖体进入位点（IRES）区域的引物以及针对SVDV RNA IRES区域高度保守区域的TaqMan探针。该检测方法在1972 - 1973年和1992年期间在欧洲分离的所有测试SVDV存档菌株以及从实验感染猪采集的临床样本中均检测到病毒RNA。rRT-PCR可以提供定量和定性信息，并且比传统RT-PCR更灵敏、执行速度更快。
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